Developments of ultra-sensitive analysis of lipid oxidation and evaluation of antioxidant actibity by chemiluminescence

脂质氧化超灵敏分析及化学发光抗氧化活性评价研究进展

• 批准号: 61850150
• 负责人: NIKI Etsuo
• 金额: $ 1.6万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61850150/
• 关键词: chemiluminescence; hydroperoxide; HPLC; lipid peroxide; 高速液体クロマトグラフィー; 自動酸化; 酸化劣化; 抗酸化剤

The objectives of the present study were to develop a sensitive assay for determination of oxidation and also for evaluation of antioxidant activity. The thermal and redical-induced decomposition of hydroperoxides yieled chemiliminescence, but the quantitative analysis was difficult. The addition of sodium hypochlorite to a solution containing a small amount, of hydroperoxide oroduced a strong chemiluminescence. This method was quite sensitive and could be applied for assessing to oxidation deterioration of oils and fats.Furthormore a new method for the detectrion of various lipid hydroperoxides and bydrogen preoxide at the picomole level has been developed by combining an HPLC system with an ultrasensitive analytical system besed on the detection of chemiluminescence emitted by isoluminol in the presence of hydroperoxide and microperoxidase. The addition of antioxidant to a chemiluminescence emitting medium, its intensity dectrased matkedly and after showing a minimum, it increased again to the initial level after some duration of time. The antioxidant activity could be evaluated from the above minimum intensity and duration of time. Thus, chemiluminescence method was found to be quick useful and applied.

本研究的目的是开发一种灵敏的测定方法来测定氧化并评估抗氧化活性。氢过氧化物的热和氧化诱导分解产生化学发光，但定量分析很困难。将次氯酸钠添加到含有少量氢过氧化物的溶液中会产生强烈的化学发光。该方法灵敏度高，可用于油脂氧化变质的评价。此外，以异鲁米诺在氢过氧化物和氢过氧化物存在下发出的化学发光检测为基础，将高效液相色谱系统与超灵敏分析系统相结合，建立了皮摩尔水平上检测各种脂质氢过氧化物和预氧化氢的新方法。 微过氧化物酶。在化学发光介质中添加抗氧化剂后，其强度急剧下降，出现最低值后，经过一段时间后又恢复到初始水平。抗氧化活性可以根据上述最小强度和持续时间来评价。因此，化学发光方法被发现是快速有用和应用的。

项目成果

山本順寛,金田尚志,植田伸夫 編: "過酸化脂質実験法" 医歯薬出版, 245 (1987)

山本淳弘、金田隆、上田伸夫（编）：《脂质过氧化物实验方法》石药出版社，245（1987）

E.Niki: J.Japan Petrol.Inst.30. 369-377 (1987)

E.Niki：J.Japan Petrol.Inst.30。

Y. Yamamoto: Ishiyakn Shuppan. Experiments for Lipid Peroxides, 238-241 (1987)

Y. Yamamoto：Ishiyakn Shuppan。

Y.Yamamoto: Anal.Biochem.160. 7-13 (1987)

Y.Yamamoto：Anal.Biochem.160。

Y.Yamamoto: Biol.Med.3. 359-361 (1987)

Y.Yamamoto：Biol.Med.3。