Developments of ultra-sensitive analysis of lipid oxidation and evaluation of antioxidant actibity by chemiluminescence

本研究的目的是建立一种灵敏的测定氧化和评价抗氧化活性的方法。氢过氧化物的热分解和自由基诱导分解产生化学发光，但定量分析困难。在含有少量过氧化氢的溶液中加入次氯酸钠会产生强烈的化学发光。该方法灵敏度高，可用于油脂氧化变质的评价。此外，将高效液相色谱系统与基于检测异亮醇在过氧化氢和微过氧化物酶存在下发出的化学发光的超灵敏分析系统相结合，建立了一种在皮摩尔水平上检测各种脂质氢过氧化物和预氧化氢的新方法。在化学发光介质中加入抗氧化剂后，其发光强度明显下降，在达到最小值后，一段时间后又恢复到初始水平。抗氧化活性可从上述最小强度和持续时间来评价。因此，发现化学发光法是一种快速、实用的方法。

The objectives of the present study were to develop a sensitive assay for determination of oxidation and also for evaluation of antioxidant activity. The thermal and redical-induced decomposition of hydroperoxides yieled chemiliminescence, but the quantitative analysis was difficult. The addition of sodium hypochlorite to a solution containing a small amount, of hydroperoxide oroduced a strong chemiluminescence. This method was quite sensitive and could be applied for assessing to oxidation deterioration of oils and fats.Furthormore a new method for the detectrion of various lipid hydroperoxides and bydrogen preoxide at the picomole level has been developed by combining an HPLC system with an ultrasensitive analytical system besed on the detection of chemiluminescence emitted by isoluminol in the presence of hydroperoxide and microperoxidase. The addition of antioxidant to a chemiluminescence emitting medium, its intensity dectrased matkedly and after showing a minimum, it increased again to the initial level after some duration of time. The antioxidant activity could be evaluated from the above minimum intensity and duration of time. Thus, chemiluminescence method was found to be quick useful and applied.