Development of a new biotechnological manufacturing process of bifidogenic substances in milk

牛奶中双歧物质的新生物技术制造工艺的开发

• 批准号: 61860030
• 负责人: ADACHI Susumu
• 金额: $ 5.12万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61860030/
• 关键词: Bifidus factor; Trichoderma harzianum; 6'-galactosyl-lactlose; ビフィダス因子; トリコデルマ; ビフィダス活性; 6'-ガラクトシル・ラクトース

Trichoderma harzianum produces galacto-oligosaccharides particularly tri- saccharides during growth in lactose containing medium. 6'-Galactosyl-lactgose is the main oligosaccharide formed by Trichoderma harzianum. In its active state, the <beta>-galactosidase isolated from Trichoderma harzianum shows signi- ficant transgalactosylation activity. The Trichoderma harzianum strain used in the presen tinvestigation is ghghly capable of forming <beta>(1-6) linkage which in turn leads to high amount production of 6'-galactlosyl-lactgose. It is thorght that the present strain of Trichoderma harzianum could be further studied critically to formulate optimum process conditions for use in large scale production of bifidogenic oligosaccharides particularly 6'-galactosyl- -lactose from lactose.

哈茨木霉在含乳糖的培养基中生长期间产生低聚半乳糖，特别是三聚半乳糖。6 '-半乳糖基-乳糖是哈茨木霉产生的主要寡糖。从<beta>哈茨木霉中分离的β-半乳糖苷酶在活性状态下具有显著的转半乳糖基活性。哈茨木霉（Trichoderma harzianum）是一株能形成<beta>（1-6）键的高产6 ′-半乳糖基乳糖的菌株。认为本菌株可进一步研究，以制定最佳工艺条件，用于从乳糖大规模生产生物合成寡糖，特别是6 ′-半乳糖基-β-乳糖。

项目成果

Sai Prakash, Kyozo Suyama, Takatoshi Itoh, Susumu Adachi: "Oligoaccharide formation by Tgrichoderma harzianum in lactose containing medium." Biotechnology Letters. 9. 249-252 (1987)

Sai Prakash、Kyozo Suyama、Takatoshi Itoh、Susumu Adachi：“哈茨木霉在含乳糖培养基中形成寡糖。”

Sai Prakash B;Kyozo Suyama;Takatoshi Itoh;Susumu Adachi: Biotechnology.

Sai Prakash B；Kyozo Suyama；Takatoshi Itoh；Susumu Adachi：生物技术。

Sai Prakash; Kyojo Suyama; Takatoshi Itoh, and Susumu adachi: Biote chnolojy Letters. 9. 249-252 (1987)

赛·普拉卡什；