Development of a new biotechnological manufacturing process of bifidogenic substances in milk

哈茨木霉在含乳糖的培养基中生长期间产生低聚半乳糖，特别是三聚半乳糖。6 '-半乳糖基-乳糖是哈茨木霉产生的主要寡糖。从<beta>哈茨木霉中分离的β-半乳糖苷酶在活性状态下具有显著的转半乳糖基活性。哈茨木霉（Trichoderma harzianum）是一株能形成<beta>（1-6）键的高产6 ′-半乳糖基乳糖的菌株。认为本菌株可进一步研究，以制定最佳工艺条件，用于从乳糖大规模生产生物合成寡糖，特别是6 ′-半乳糖基-β-乳糖。

Trichoderma harzianum produces galacto-oligosaccharides particularly tri- saccharides during growth in lactose containing medium. 6'-Galactosyl-lactgose is the main oligosaccharide formed by Trichoderma harzianum. In its active state, the <beta>-galactosidase isolated from Trichoderma harzianum shows signi- ficant transgalactosylation activity. The Trichoderma harzianum strain used in the presen tinvestigation is ghghly capable of forming <beta>(1-6) linkage which in turn leads to high amount production of 6'-galactlosyl-lactgose. It is thorght that the present strain of Trichoderma harzianum could be further studied critically to formulate optimum process conditions for use in large scale production of bifidogenic oligosaccharides particularly 6'-galactosyl- -lactose from lactose.