On formation and chemical structure of functional oligasaccharide from lactose

乳糖功能性低聚糖的形成及其化学结构

• 批准号: 59430022
• 负责人: ADACHI Susumu
• 金额: $ 13.57万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1985
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59430022/
• 关键词: galacto-oligosaccharire; <beta> -galactosidase; ラクトース

Di-, tri-, tetra-, penta- and hexasaccharides were formed during the hydrolysis of lactose by transgalactosylation reaction of Aspergillus oryzae <beta> -galactosidase. In this study the isolation and characterization of the major constituents of tri-, tetra- and pentasaccharides are described. The structure elucidation of 3 tri-, 2 tetra- and 1 pentasaccharides was carried out by methylation analysis, mass spectrometry and <^(13)C> -nmrspectrometry. The trisaccharides are 3'-galactosyl-lactose, 6'-galactosyl -lactose and 4,6-digalactosyl-glucose. Tetrasaccharides are O- <beta> -D-galacto pyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-4)-D-glucose and O- <beta> -D-galactopyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-3) [or O- <beta> -D-galactopyranosyl-(1-3)-O- <beta> -D-galactopyranosyl-(1-6)]-O- <beta> -D-galactopyranosyl-(1-4)-D-glucose. Pentasaccharide is O- <beta> -D-galactopyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-4)-D-glucose.

在米曲霉β-半乳糖苷酶的转半乳糖基化反应的乳糖水解过程中形成二糖、三糖、四糖、五糖和六糖。在这项研究中，描述了三糖、四糖和五糖主要成分的分离和表征。通过甲基化分析、质谱分析和<^(13)C>-核磁共振分析对3种三糖、2种四糖和1种五糖进行了结构解析。三糖是3'-半乳糖基-乳糖、6'-半乳糖基-乳糖和4,6-二半乳糖基-葡萄糖。四糖是O-<β>-D-吡喃半乳糖基-(1-6)-O-<β>-D-吡喃半乳糖基-(1-6)-O-<β>-D-吡喃半乳糖基-(1-4)-D-葡萄糖和O-<β>-D-吡喃半乳糖基-(1-6)-O-<β> -D-吡喃半乳糖基-(1-3)[或O-β-D-吡喃半乳糖基-(1-3)-O-β-D-吡喃半乳糖基-(1-6)]-O-β-D-吡喃半乳糖基-(1-4)-D-葡萄糖。五糖是O-β-D-吡喃半乳糖基-(1-6)-O-β-D-吡喃半乳糖基-(1-6)-O-β-D-吡喃半乳糖基-(1-4)-D-葡萄糖。

项目成果

Food Chemistry. 16-2. (1985)

食品化学。

Japanese Journal of Dairy and Food Science. 34-6. (1985)

日本乳制品和食品科学杂志。

酪農科学・食品の研究. 34-6. (1985)

乳制品科学和食品研究。34-6。