Preparative method of functional oligasaccharides from bovine colostrum

牛初乳功能性低聚糖的制备方法

• 批准号: 59860031
• 负责人: ADACHI Susumu
• 金额: $ 4.03万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1985
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-59860031/
• 关键词: caseinoglycopeptide; <kappa> -casein; bovine colostrum; oligosaccharide; ビフィドバクテリウム活性

Colostrum was taken from a Holstein-Friesian cow 6h after parturition. Isolation of milk sugars from defatted colostrum by dialysis was fermormed by the method of Schneir and Rafelson. The complete removal of peptides from the dialyzate was achieved by ion-exchange chromatography with Sp-Sephadex C-50 by our earlier method. The sugars remaining after removal of lactose by crystallization were fractionated by gel filtration chromatography(Bio-gel P-2) with distilled water as eluent. On the otherhand, the colostrum glycopeptide was treated with NaOH of NaB <H_4> for 36h at 50 C in the dark under <N_2> , followed by removal of low-molecular peptides and salts using Dowex 50W-X4 resin. The resulting oligosacchariditols were finally purified by gel filtration chromatography(Biogel P-2) with distilled water as eluent. The purified oligosaccharditols were fractionated by ion-exchange chromatography using the method of Farrar et al in a linear concentration gradient of 0.05-1.0M of pyridine-acetate buffer.

初乳取自荷斯坦-弗里斯兰奶牛，产后 6 小时。采用 Schneir 和 Rafelson 的方法通过透析从脱脂初乳中分离乳糖进行发酵。我们之前的方法通过使用 Sp-Sephadex C-50 的离子交换色谱法实现了从透析液中完全去除肽。通过结晶除去乳糖后剩余的糖通过凝胶过滤色谱法(Bio-gel P-2)以蒸馏水作为洗脱液分级分离。另一方面，将初乳糖肽用NaB <H_4>的NaOH在<N_2>下于50℃避光处理36小时，然后使用Dowex 50W-X4树脂去除低分子肽和盐。最后通过凝胶过滤色谱法(Biogel P-2)以蒸馏水作为洗脱液纯化所得低聚糖醇。使用Farrar等人的方法，在0.05-1.0M吡啶-乙酸盐缓冲液的线性浓度梯度中，通过离子交换色谱法对纯化的低聚糖进行分级。

项目成果

Biochimica et Biophysica Acta. 801-. (1984)

生物化学与生物物理学学报。

Biochimica et Biophysica Acta. 801. (1984)

生物化学与生物物理学学报。