Preparative method of functional oligasaccharides from bovine colostrum

初乳取自荷斯坦奶牛分娩后6 h。本文采用Schneir和Rafelson的方法，对脱脂初乳中乳糖的透析分离进行了研究。从透析液中完全去除肽是通过我们早期的方法用Sp-Sephadex C-50离子交换色谱实现的。通过凝胶过滤色谱法（Bio-gel P-2），用蒸馏水作为洗脱剂，分级分离通过结晶除去乳糖后剩余的糖。另一方面，初乳糖肽用NaB的NaOH<H_4>在50 ℃下在黑暗中处理36小时<N_2>，然后用Dowex 50 W-X4树脂除去低分子肽和盐。最终通过凝胶过滤色谱法（Biogel P-2）用蒸馏水作为洗脱剂纯化所得寡糖二醇。使用Farrar等人的方法，在0.05-1.0M的吡啶-乙酸盐缓冲液的线性浓度梯度中，通过离子交换色谱法分级分离纯化的寡糖二醇。

Colostrum was taken from a Holstein-Friesian cow 6h after parturition. Isolation of milk sugars from defatted colostrum by dialysis was fermormed by the method of Schneir and Rafelson. The complete removal of peptides from the dialyzate was achieved by ion-exchange chromatography with Sp-Sephadex C-50 by our earlier method. The sugars remaining after removal of lactose by crystallization were fractionated by gel filtration chromatography(Bio-gel P-2) with distilled water as eluent. On the otherhand, the colostrum glycopeptide was treated with NaOH of NaB <H_4> for 36h at 50 C in the dark under <N_2> , followed by removal of low-molecular peptides and salts using Dowex 50W-X4 resin. The resulting oligosacchariditols were finally purified by gel filtration chromatography(Biogel P-2) with distilled water as eluent. The purified oligosaccharditols were fractionated by ion-exchange chromatography using the method of Farrar et al in a linear concentration gradient of 0.05-1.0M of pyridine-acetate buffer.