Microscopic image processing system based on personal microcomputer

开发了一种方便的彩色图像处理系统，它包括从日本供应商那里容易获得的必要的5个相关部件：CCD彩色电视摄像机(IK1570，Nakamura Science Co.)、自制的简化宽带视频放大器、高速彩色图像数据采集接口板(Nippon Board Computer Co.)、彩色视频监视器和16位个人微型计算机系统(PC-9801Vm2，NEC公司，Lts.)利用高分辨率RGB终端显示器(640x400点)、彩色打印点打印机和称为鼠标的坐标数据输入设备，将放大的视频信号送入图像处理板，以1/60秒的速度对256x256像素的每个RGB 6位二进制数据进行数字化，并将数据存储在板上的存储器中。为了在高分辨率RGB显示器上显示处理后的图像数据，微型计算机将分级密度的ON像素数据分别转换为R、G、B平面的5个分级2.2点图案。用N88 Disk BASIC(86)和机器语言子程序开发了图像SATA的采集、显示、控制和测量软件。应用该彩色图像处理系统，通过玻璃微电极向培养的小鼠神经母细胞(NIE 115)细胞内注入外源荧光染料(7%荧光黄)获得两种不同的荧光显微图像数据，并用组织化学方法检测鲤鱼视网膜内源性多巴胺荧光。实验结果表明，采用宽带视频信号放大、色密度转换和叠加等方法对同一样品采集的多幅图像的图像数据进行处理，可以有效地在微弱荧光显微图像上从模糊的环境中恢复出细胞体的形状。

A convenient color image processing system was developed consisting essential 5 interrelated components which were obtained easily from the vendor in Japan, CCD color TV camera (IK 1570, Nakamura Scientific Co. Ltd.), homemade simplified wideband video amplitier, high speed color image data acquisition interface board (Nippon Board Computer Co. Ltd.), color video monitor and 16 bits personal microcomputer system (PC-9801Vm2, NEC Co., Lts.) with the high resolutional RGB terminal display (640x400 dots), color dot printer for hard copy and the coordinate data input equipoent called mouse, Amplitied video signal is fed into the image processing board to digitize 256x256 pixels with each RGB 6 bits binary data at 1/60 secons and to store the data in the memories on the board. In order to display the image data processed on the high resolution RGB monitor, mictocomputer converts the on pixel data with 64 graded density to 5 graded 2.2 dots pattern of the R, G, and B plane, respectively. Software has been dfeceloped by N88 DISK BASIC (86) and machine language subroutine for acquiring, displaying, controlling and measuring the image sata. This color image processing system was applied to two different fluorescent microscopic image data which were obtained by the intracellular injection of exogenous fluorescent dye (7% Lucifer Yellow) through the glass mictoelectrode into the cultured mouse neuroblastome (NIE 115) cells and by the histochemical methods to detect the endogenout dopamine dluorescence in the carp retina. Our experimental results show that the wideband video signal amplifier, color density conversion methods and adding methods to the image data of many pictures acquired from the same preparation are very effective to rise up shape of cell body from obscure surroundings on the weak fluorescent microscopic image.