Identification of regeneration-associated genes in zebrafish by a ChIP-on-chip assay and its application to mammals.

通过芯片上 ChIP 测定鉴定斑马鱼再生相关基因及其在哺乳动物中的应用。

• 批准号: 22300109
• 负责人: KATO Satoru
• 金额: $ 11.48万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22300109/
• 关键词: 神経再生分子; HSP70; エピジェネティクス; HDACインヒビター; 神経生存; 軸索伸長; CHIPアッセイ; 転写因子; NO; HDAC-2; S-ニトロシル化; RARβ; 軸索再伸長; 魚; 視神経再生; 細胞生存; ラット; ゼブラフィッシュ; Hsp70; 最初期遺伝子; 網膜神経節細胞; 細胞生子

Regeneration-associated genes (RAGs) were cloned from axotomized zebrafish retina. HSP70 (a stress protein) and purpurin (a retinol-binding protein) were rapidly induced in the fish retina after optic nerve injury. HSP70 was involved in cell survival of injured retinal ganglion cells (RGCs), whereas purpurin was involved in neurite sprouting from injured RGCs. These RAGs could easily induce regrowth of axotomized optic nerve in adult rat.

从斑马鱼视网膜中克隆了再生相关基因（RAGs）。视神经损伤后，鱼视网膜快速诱导应激蛋白HSP70和视黄醇结合蛋白purpurin。HSP70参与损伤视网膜神经节细胞（RGCs）的细胞存活，而purpurin参与损伤视网膜神经节细胞的神经突萌发。这些破布能很容易地诱导成年大鼠视神经的再生。

项目成果

A novel function of Factor XIII-A in nerve regeneration

XIII-A 因子在神经再生中的新功能

• 发表时间: 2013
• 作者: Hayashi;Y.;Sugitani K
• 通讯作者: Sugitani K

HSP70 Gene Expression in the Zebrafish Retina After Optic Nerve Injury: A Comparative Study Under Heat Shock Stresses

• DOI: 10.1007/978-1-4614-0631-0_84
• 发表时间: 2012-01-01
• 期刊: RETINAL DEGENERATIVE DISEASES
• 作者: Fujikawa, Chieko;Nagashima, Mikiko;Kato, Satoru
• 通讯作者: Kato, Satoru

Reciprocal changes of factor XIII and retinal transglutaminase expressions in the fish retina during optic nerve regeneration

视神经再生过程中鱼视网膜中因子XIII和视网膜转谷氨酰胺酶表达的相互变化

• 发表时间: 2012
• 作者: Nihei Y;Ito D;Okada Y;Akamatsu W;Yagi T;Yoshizaki T;Okano H;Suzuki N.;杉 谷 加 代
• 通讯作者: 杉 谷 加 代

A distinct effect of two types of transglutaminase on fish optic nerve regeneration

两种转谷氨酰胺酶对鱼类视神经再生的不同作用

• 发表时间: 2012
• 作者: Furumoto T;Fujita K;Toyoshima M;Zaiki K;Sahara S;Akita M;Ozawa N;Nakaoji K;Hamada K;Tamai K;Kaneda Y;Maeda A.;杉 谷 加 代
• 通讯作者: 杉 谷 加 代

Protective action of nipradilol mediated through S-nitrosylation of Keap1 and HO-1 induction in retinal ganglion cells

• DOI: 10.1016/j.neuint.2012.09.004
• 发表时间: 2012-12
• 期刊: Neurochemistry International
• 影响因子: 4.2
• 作者: Y. Koriyama;M. Kamiya;T. Takadera;K. Arai;K. Sugitani;K. Ogai;S. Kato