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Behavioral analysis of fish activity by a computer image processing system

利用计算机图像处理系统对鱼类活动进行行为分析

基本信息

批准号：
07558105
负责人：
KATO Satoru
金额：
$ 0.64万
依托单位：
KANAZAWA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

We developed a computer image processing system to acquire positional coordinates of goldfish freely moving in an aquarium. The system was composed of two CCD cameras and two interface I/O boards. The output was fed into an IBM PC/AT compatible computer. By using this image processing system, we could get a trace map of moving goldfish on real time for a long period. Furthermore, we constructed various analytical histograms (moving velocity, right or left turning, inclination of body axis) to quantitatively score fish behavior. We applied these analyzes for the goldfish behavior after treatment of hemisection of the optic nerve. The experimental procedure induced abnormal turning and inclinating behaviors toward the intact side. This abnormality continued for 7-10 days and then were gradually recovered to control fish until 30-40 days after optic nerve injury. To confirm regeneration of the optic nerve, a tracer HRP was injected to one eye, in which the optic nerve had been hemisectioned, and optic nerve terminals in the optic tectum were followed after treatment. The retino-tectal connection of treated goldfish was completed like control by 30 days after hemisection. The regenerating optic nerve was also quantified by ^3H-proline labeling method. These results indicate complete regeneration of the goldfish optic nerve after hemisection at a cellular and a behavioral level. The all different methods described above were good correlated each other. Therefore, the computer image processing system is a very useful quantitation tool for fish behavior and is applicable to the other field of fish science.

我们开发了一个计算机图像处理系统来获取金鱼在水族箱中自由运动的位置坐标。该系统由两台CCD摄像机和两块接口I/O板组成。将输出输入IBM PC/AT兼容计算机。利用该图像处理系统，我们可以得到真实的长时间运动的金鱼的轨迹图。此外，我们构建了各种分析直方图（移动速度，右或左转向，身体轴的倾斜度），以定量评分鱼的行为。我们将这些分析应用于治疗视神经半切后的金鱼行为。实验过程诱导异常转向和倾斜的行为向完整的一面。这种异常持续7-10天，然后逐渐恢复到对照鱼，直到视神经损伤后30-40天。为了确认视神经的再生，将示踪剂HRP注射到其中视神经已被半切的一只眼睛中，并在治疗后跟踪视顶盖中的视神经末梢。半切后30天，处理组金鱼的视网膜-顶盖连接与对照组一样完成。用^3H-脯氨酸标记法对再生视神经进行定量。这些结果表明在细胞和行为水平上，半切后金鱼视神经完全再生。上述所有不同的方法彼此之间具有良好的相关性。因此，计算机图像处理系统是一个非常有用的定量工具，鱼类行为，并适用于其他领域的鱼类科学。