Development of diagnostic methods for acute myocordial infarction using monoclonal antibody

判断急性心肌梗死的范围和部位对判断预后和制定治疗方案具有重要意义。已知单克隆抗体具有高度特异性和均一性，可以大量生产。根据这些特点，我们开发了一种新的诊断心肌梗死的方法，具有良好的特异性和定量。由于心肌肌球蛋白是心肌特异性的主要结构蛋白，特别是其轻链和重链亚基具有不同的性质，我们制备了相应的单克隆抗体。利用这些特异于重链的抗体与梗塞心肌的肌原纤维结合而聚集的事实，我们通过实验证明，通过静脉注射同位素标记的单克隆抗体可以获得高度特异性和清晰的心肌梗塞图像。我们还建立了一个非常敏感的微量测定使用抗体，特异性结合轻链释放的心肌细胞进入血清followin缺血事件，并开发了一种方法，用于生化诊断心肌梗死。现在我们可以获得准确的信息，梗塞的大小，这将是不可能的，否则通过传统的方法，广泛的临床应用，预计这种方法。

Estimating the size and location of infarct is important in predicting the prognosis and determining the therapeutic strategy for patients with acute myocardial infarction. Monoclonal antibodies are Known to be highly specific and uniform and can be produced in quantity. Based on these characteristics, we developed a new diagnostic method for myocardial infarction which has excellent specificity and quantitativeness. Noting that cardiac myosin is a major structural portein specific to the myocardium and especially its subunits, light chain and hevay chain, have different properties, we prepared corresponding monoclonal antibodies. Utilizing the fact that these antibodies specific for heavy chain accumulate bound to the myofibril of the infarcted myocardium, we demonstrated through experiments that highly specific and clear image of myocardial infarction can be obtainedby intravenous administration of isotope labelled monoclonal antibodies. We also established a very sensitive microassay using antibodies which specifically bind the light chains liberated from the myocyte into serumfollowin ischemic events, and developed a method for biochemically diagnosing myocaridial infarction. Now that we can obtain accurate information regarding the size of the infarct which would be otherwise impossible by the conventional method, a wide range of clinical application is expected for this method.