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魚類における産卵期調節法の確立と染色体工学への適用

鱼类产卵期调控方法的建立及其在染色体工程中的应用

基本信息

批准号：
63860025
负责人：
会田勝美
金额：
$ 6.85万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

1.環境条件操作およびホルモン投与による産卵期調節法の確立a)クロダイ雌親魚1尾と雄親魚2ー3尾を自然水温および産卵適温に制御した屋内水槽(3.6x1.6x0.8m)に収容し、産卵期の長期化が可能であるか検討した。自然水温下では4月下旬から7月上旬まで産卵したが水温制御下(18.5ー20.5℃)では半月程産卵期が延長した。またオゾン通気した水温制御水槽では8月15日まで産卵が継続した。またトビヌメリの場合も産卵適温を維持すると長期間に渡り産卵を繰り返し続けることが判明した。b)キンギョの場合水温上昇刺激により春産卵期の早期化が可能であり、また長日下15ー20℃の中間温度帯で飼育を続けると長期間に渡って産卵を繰り返すことが判明した。ホンモロコの場合も水温上昇のみで春産卵期の早期化が可能であり、また秋に高温・長日により再熟を誘導できることが判明した。c)定時産卵現象を示すトビヌメリの産卵時刻は光周期により決定されることが判明した。d)これまで大型回遊魚であるブリの池中での催熟・産卵誘発は困難であったが、LHRHアナログのペレット投与により産卵誘発に成功した。2.染色体操作による倍数体の作出a)他のサケ科魚より産卵時期の早いヤマメの産卵期を光周期調節により遅延させ、異質倍数体の作出を行った。その結果、産卵期調節と染色体操作によりヘテロシスと不妊化の両メリットを併せ持つ新しい異質倍数体が得られることが判明した。b)受精後の低温処理によりマダイ3倍体魚の大量作出に成功した。

1. The environmental condition operation is related to the establishment of a) the natural water temperature of the tail, the natural water temperature of the male, the natural water temperature of the tail, the temperature of the eggs, the environmental conditions, the environmental conditions and the environmental conditions. Under the natural water temperature, the water temperature (18.5-20.5 ℃) and the half-month egg period (18.5-20.5 ℃) were measured in late April and early July. The temperature of the water should be controlled in the sink. On August 15, the eggs would be closed. Please ensure that the temperature of the egg is maintained for a long period of time. B) it is possible to stimulate the early stage of spring eggs at the temperature of the water, and the temperature between 15 ℃ and 20 ℃ for a long time may determine the recovery rate of eggs. The temperature of the water is very high, and the early stage of the spring egg stage may be affected by the high temperature in autumn and the reripening of the egg in the autumn. C) the photoperiod response is determined by the photoperiod response at the same time as the time-lapse egg image indicates that the photoperiod is detected. D) the eggs in the pool were induced to mature, the eggs were trapped in the pool, and the eggs were successfully thrown in the pool. D) the eggs in the pool were not mature, the eggs were trapped, and the eggs were successful. D) the eggs in the pool were not mature, and LHRH eggs were successful. two。 Chromosome manipulation is performed by ploidy. A) the photoperiod is delayed in the early phase of ovum, the photoperiod is delayed, and the ploidy is made. The results showed that the results of chromosome manipulation during the egg period, the chromosome manipulation, the non-pregnancy, the non-pregnancy, the polyploidy, the chromosome manipulation, the pregnancy-free pregnancy, the pregnancy-free pregnancy, the polyploidy, the polyploidy, the polyploidy. B) after fertilization, a large number of triploids were made successfully after fertilization.