Production of specific pathogen-free(SPF)guinea-pigs and SPF rabbits by using gnotobiotes

SPF级家兔和SPF级豚鼠肠道菌群的控制比SPF级小鼠和大鼠更为重要，因为肠道菌群差的无菌或原无菌家兔的盲肠体积会变得非常大，对肠道感染非常敏感。在本研究中，我们尝试生产原gf兔和原gf豚鼠，并建立SPF菌落。无菌（GF）兔接种6株拟杆菌（B株），B株接种2株链球菌（B+S），从10^<-5>稀释的普通（CV）兔盲肠内容物（CF）或接种CV兔CF的前GF小鼠培养菌群，以及氯仿处理的CV兔盲肠内容物（CHF）中培养菌群。GF家兔出生3周后口服B株或B+S， 1天后口服CF或CHF。接种。前gf家兔接种B株和CF后成活率均在90%以上，体重增加与CV家兔相似。相反，只接种CF或只接种B株的原gf兔在接种后3周内全部死亡。B+S和CHF接种组死亡率分别为50%和20%。但是，它们的增重和健康状况都不理想，CV兔的CF与接种CV兔CF的前GF小鼠的CF对GF兔的影响没有差异。在豚鼠的情况下，芽孢杆菌或双歧杆菌的组合为第一。从CV豚鼠接种和CF可用于建立SPF菌落的前gf豚鼠。

Control of the intestinal flora of SPF rabbits and SPF guineapigs are more important than that of SPF mice and rats, because the cecal size of germfree(GF) or ex-GF rabbits with poor intestinal flora become very large, and they are sensitive against enteric infections. In this study, we attempted to produce ex-GF rabbits and ex-GF guineapigs, and to establish SPF colonies.Germfree(GF) rabbits were inoculated with 6 strains of Bacteroides(B strains), B strains with 2 strains of Streptococcus(B+S), cultured flora from 10^<-5> dilution of cecal contents(CF) from a conventional(CV) rabbit or ex-GF mice inoculated with CF of CV rabbit, and chloroform-treated cecal contents of CV rabbit(CHF). GF rabbits were orally inoculated with B strains or B+S 3 weeks after birth, and then they were inoculated with CF or CHF 3 days after 1st. inoculation.Ex-GF rabbits inoculated with B strains and CF were survived at the rate of more than 90% and an increase of their body weight were similar to CV rabbits. On the contrary, all of ex-GF rabbits inoculated with only CF or with only B strains were dead within 3 weeks after inoculation. In B+S and CHF inoculated groups, mortality rate were 50% and 20%, respectively. However, weight gain and health condition of them were unsatisfactory, there were no differences on the effects to GF rabbits between CF from a CV rabbit and CF from ex-GF mice inoculated with CF from a CV rabbit.In case of guinea-pigs, the combination of Bacillus or Bifidobacterium as 1st. inoculation and CF from a CV guinea-pig was available for an establishment of ex-GF guinea-pigs for a SPF colony.