Production of specific pathogen-free(SPF)guinea-pigs and SPF rabbits by using gnotobiotes

利用侏儒菌生产无特定病原体（SPF）豚鼠和SPF兔

• 批准号: 63880011
• 负责人: MITSUOKA Tomotari
• 金额: $ 6.72万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63880011/
• 关键词: Gnotobiote; SPF guimea-pig; SPF rabbit; ノートバイオート

Control of the intestinal flora of SPF rabbits and SPF guineapigs are more important than that of SPF mice and rats, because the cecal size of germfree(GF) or ex-GF rabbits with poor intestinal flora become very large, and they are sensitive against enteric infections. In this study, we attempted to produce ex-GF rabbits and ex-GF guineapigs, and to establish SPF colonies.Germfree(GF) rabbits were inoculated with 6 strains of Bacteroides(B strains), B strains with 2 strains of Streptococcus(B+S), cultured flora from 10^<-5> dilution of cecal contents(CF) from a conventional(CV) rabbit or ex-GF mice inoculated with CF of CV rabbit, and chloroform-treated cecal contents of CV rabbit(CHF). GF rabbits were orally inoculated with B strains or B+S 3 weeks after birth, and then they were inoculated with CF or CHF 3 days after 1st. inoculation.Ex-GF rabbits inoculated with B strains and CF were survived at the rate of more than 90% and an increase of their body weight were similar to CV rabbits. On the contrary, all of ex-GF rabbits inoculated with only CF or with only B strains were dead within 3 weeks after inoculation. In B+S and CHF inoculated groups, mortality rate were 50% and 20%, respectively. However, weight gain and health condition of them were unsatisfactory, there were no differences on the effects to GF rabbits between CF from a CV rabbit and CF from ex-GF mice inoculated with CF from a CV rabbit.In case of guinea-pigs, the combination of Bacillus or Bifidobacterium as 1st. inoculation and CF from a CV guinea-pig was available for an establishment of ex-GF guinea-pigs for a SPF colony.

SPF兔和SPF豚鼠的肠道菌群控制比SPF小鼠和大鼠更重要，因为肠道菌群较差的无菌(GF)或前GF兔盲肠变得很大，而且它们对肠道感染很敏感。将6株拟杆菌(B株)、B株和2株链球菌(B+S)接种于无菌(GF)兔体内，培养出10^-lt；-5&gt的菌群；用CV兔或前GF小鼠盲肠内容物(CF)进行稀释，并用氯仿处理的CV兔盲肠内容物(CHF)。出生后3周分别口服B毒株或B+S，1日龄后3d再接种CF或CHF。接种：接种B株和CF的前GF兔成活率达90%以上，体重增长与CV兔相似。反之，单独接种CF或仅接种B株的前GF兔在接种后3周内全部死亡。B+S组和CHF组小鼠死亡率分别为50%和20%。然而，它们的体重增长和健康状况并不令人满意，CV兔的CF与前GF小鼠的CF接种CV兔的CF的效果没有差异。对于豚鼠，以芽孢杆菌或双歧杆菌的组合为第一。建立SPF菌落的前GF豚鼠，可接种一只CV豚鼠的CF值。

项目成果

Itoh,K.: Laboratory Animals.

Itoh,K.：实验动物。

Itoh,K.Sudo,K.Tanaka,T.Nakagawa,M.and Mitsuoka,T.: "Establishment of specific pathogen-free(SPF)rabbits by inoculation of hysterectomy-derived animals with cecal microlova obtained from conventional rabbits." Laboratory Animals.

Itoh,K.Sudo,K.Tanaka,T.Nakakawa,M. 和 Mitsuoka,T.：“通过用从常规兔子获得的盲肠微虫接种子宫切除术衍生的动物来建立无特定病原体 (SPF) 兔子。”

Hirayama, K., Kawamura, S., Mitsuoka, T., and Tashiro, K.: "The faecal flora of the giant panda(Ailuropcdu melamoleuca)" J. of Applied Bacteriology. 67. 411-415 (1989)

Hirayyama, K.、Kawamura, S.、Mitsuoka, T. 和 Tashiro, K.：“大熊猫的粪便菌群（Ailuropcdu melamoleuca）”《应用细菌学杂志》。

Itoh, K.: "Production of the Standard intestinal flora for experimental animals raised under parrier sustained conditions." Experimental Animals. 39. 1-8 (1990)

Itoh, K.：“为在帕里尔持续条件下饲养的实验动物生产标准肠道菌群。”

Mitsuoka, T.: "Intestinal flora and host." Asian Med.31. 400-409 (1988)

Mitsuoka, T.：“肠道菌群和宿主。”