Identification and characterization of a barley yellow dwarf virus resistance gene in maize

玉米大麦黄矮病毒抗性基因的鉴定和表征

• 批准号: 403095468
• 负责人: Dr. Antje Habekuß
• 金额: --
• 依托单位: Institut für Resistenzforschung und Stresstoleranz
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/403095468?language=en
• 关键词: Identification; characterization; barley; yellow; dwarf

Barley yellow dwarf is the most widely spread viral disease of cereals. It is caused by the Barley yellow dwarf virus (BYDV) and the Cereal yellow dwarf virus (CYDV), where the first is causing more severe damages than the second. Maize plays an important role in the epidemiology of BYDV and is as well damaged by the virus. With increasing global temperatures, viral diseases in general and BYDV in specific are expected to become prominent problems in cereal and maize cultivation. The control of the virus itself is not possible and insecticides to control the BYDV-transmitting aphids are not certificated in Germany. Resistant maize can break the transmission cycle of BYDV and therewith could as well contribute to a reduction of the BYDV problems in other cereals. In an earlier study, we detected a BYDV resistance factor at the bottom of chromosome ten, which explained considerable proportions of the phenotypic variance of the traits virus concentration and disease symptoms. Based on these experiments, the gene GRMZM2G018027 is the best candidate as BYDV resistance gene. The homolog of this gene in Arabidopsis thaliana is OXS3 that is described to be involved in the production of H2O2. As H2O2 is known to play an important role in pathogen defense, GRMZM2G018027 could be involved in a general pathogen defense reaction. One of the objectives of the proposed project is to identify and validate the BYDV resistance gene in maize. This will be reached with the help of five so called heterogeneous inbred families. Artificial inoculation experiments and experiments characterizing the sucking behavior of aphids on recombinant plants of the heterogeneous inbred families as well as genotypes that were created by transforming the maize inbred A188 with different alleles of GRMZM2G018027 will be used to improve our understanding of the molecular function of the BYDV resistance gene. Furthermore, we plan to quantify the contribution of the BYDV resistance gene to variation of other viral diseases.

大麦黄矮人是谷物中最广泛传播的病毒疾病。它是由大麦黄矮人病毒（BYDV）和谷物黄矮人病毒（CYDV）引起的，其中第一个造成的损害比第二次造成更严重的损害。玉米在BYDV的流行病学中起重要作用，并且该病毒也受到了损害。随着全球温度的升高，预计通常在谷物和玉米种植中成为特定问题的病毒性疾病。对病毒本身的控制是不可能的，并且在德国未获得杀虫剂来控制BYDV传播蚜虫。耐药性玉米可以打破BYDV的传输周期，因此也可能有助于减少其他谷物中的BYDV问题。在较早的研究中，我们在第十染色体的底部检测到了一个BYDV抗性因子，该因子解释了特征病毒浓度和疾病症状的表型差异的相当比例。基于这些实验，基因GRMZM2G018027是BYDV抗性基因的最佳候选者。该基因在拟南芥中的同源物是OXS3，被描述为H2O2的生产。由于已知H2O2在病原体防御中起重要作用，因此GRMZM2G018027可能参与了一般的病原体防御反应。拟议项目的目标之一是识别和验证玉米中的BYDV抗性基因。这将在五个所谓的异构近亲家庭的帮助下实现。人造接种实验和实验表征了蚜虫在异质性近交系列的重组植物上的吮吸行为以及通过转换玉米近近近近代A188创建的基因型，这些基因型将使用不同的GRMZM2G018027等位基因来改善我们对我们对分子依赖性基因的理解。此外，我们计划量化BYDV耐药基因对其他病毒疾病变异的贡献。