Exploring the functional role of Hijiki in obesity-associated to sphingolipid metabolism

探索羊栖菜在与鞘脂代谢相关的肥胖中的功能作用

• 批准号: 21K14812
• 负责人: BommeGowda SiddabasaveGowda
• 金额: $ 2.58万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K14812/
• 关键词: Hijiki; obesity; Sphingolipid metabolism; LC/MS; Obesity

The project is going well, we have identified the active fraction and component of hijiki responsible for SMS inhibition. The in vitro assays were performed using the active fraction of hijiki in C3A cells to reveal its action against lipid droplet accumulation. We have found that hijiki active fraction significantly suppressed the lipid droplet accumulation. However, these are preliminary results, we are examining in detail by various analysis including LC/MS analysis of lipid contents changed upon hijiki active fraction treatment. Further, the active fraction is purified by column chromatography after repetitive purification, HRMS and NMR data of the pure compound were obtained. The structural analysis is in progress after gathering all the data.

该项目进展顺利，我们已经确定了羊栖菜的活性馏分和组分负责SMS抑制。在C3 A细胞中使用羊栖菜的活性级分进行体外测定，以揭示其对脂滴积累的作用。我们已经发现羊栖菜活性组分显着抑制脂滴积累。然而，这些是初步结果，我们正在通过各种分析进行详细检查，包括LC/MS分析在hijiki活性部分处理后脂质含量的变化。此外，活性级分在重复纯化后通过柱色谱法纯化，获得纯化合物的HRMS和NMR数据。收集所有数据后，正在进行结构分析。

项目成果

Analysis of lipid biomarkers for heart failure by LC/MS

通过 LC/MS 分析心力衰竭的脂质生物标志物

• 发表时间: 2022
• 作者: 岸本真治;南歩実;松原有也;竹島実加;渡邉正悟;渡辺賢二;Siddabasave Gowda B. Gowda
• 通讯作者: Siddabasave Gowda B. Gowda