ふ化場魚を野生魚に近づける:環境エンリッチメントを利用したサケ稚魚の種苗性向上

让孵化场鱼类更接近野生鱼类：利用环境富集提高幼鲑鱼的苗种质量

• 批准号: 21K14908
• 负责人: 越野 陽介
• 金额: $ 2.91万
• 依托单位: Hokkaido Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K14908/
• 关键词: サケ; ふ化放流事業; 環境エンリッチメント; サケ稚魚; 飼育実験; ふ化増殖事業

現在，北日本ではサケ資源の減少が続いている。その要因の一つとして，ふ化放流事業を長い間続けてきたことにより，ふ化場魚が自然環境下での生存に不利な形質や特性を持つようになった可能性が指摘されている。近年，飼育環境を改良すること環境エンリッチメント(以下,EE)で，魚類の成長や発達が向上することが分かってきた。サケにおいても，飼育環境を本来の自然環境に近づけることでその特性を野生魚に近づけ，種苗としての質を高めることができるかもしれない。そこで2年目は，①実際のさけます増殖現場でも取り入れやすい構造物の設置試験，②さけます増殖現場での飼育試験，③EEが脳内でのシナプス可塑性へ与える影響を明らかにすることを目的とした，稚魚の飼育時におけるEEの有無による小脳でのsyp mRNA発現解析を行った。増殖現場でも取り入れやすいように，フロートや重石をロープから吊り下げるように改良した構造物を設置したEE群では，対照群(何も設置しない従来の飼育環境)よりも成長が高まる傾向がみられた。同様の構造物を実際の増殖現場に設置した場合でも，成長の向上が確認されたことから，EEによる飼育池内への構造物の設置はサケの成長を高めることが昨年に引き続き確かめられた。サケ稚魚の小脳におけるsym RNA解析の結果，飼育20日後では対照群よりも実験群の方が発現量が高いことが明らかとなった。対照群とEE群では尾叉長と同発現の間に正の相関が認められ，体サイズ増加に伴い同発現量が増加していた。このことは，成長に伴う神経可塑性の増加は通常状態でも存在するが，EE群では小脳で高い神経可塑性の状態であったことが明らかとなった。一方,40日後の結果では同様の結果が得られなかったことから，今後サンプル数や解析部位を検討しなおす必要がある。

At present, there is a shortage of tourism resources in North Japan. As a result of the emergency, the chemical release business has been in trouble for a long time, and the adverse characteristics of survival in the natural environment have been criticized for the possibility of survival in the natural environment. In recent years, the environment of education and education has improved, and the growth of education and education has reached a high level. In recent years, the environment of education has improved, and the growth of education has reached a high level. In recent years, the environment of education has been improved, the environment has improved, and the growth of education has reached a high level. The nature of the natural environment, the characteristics of the natural environment, the nature of the natural environment. In the past two years, there are two years of research, one is that the equipment of the production system is installed in the international market, the other is the facility of the plant, and the plasticity of the 3EE system is different from that of the system. The purpose of the experiment is to understand the purpose of the system, and there is an analysis in the EE system during the period of early childhood education. The plant is collected into the environment, and the heavy stone is hoisted under the crane to improve the installation of the EE group, and according to the group (why do you set the equipment to nurture the environment) the growth rate is high and the temperature is high. In conjunction with the facility of the EE creation facility, the growth rate has been confirmed upward, and the growth rate has been set up in the growth pool. The growth rate of the plant has been introduced last year. After 20 days of education, the results of the sym RNA analysis were analyzed. After 20 days of education, the results of the analysis of the results showed that after 20 days of education, the results of the analysis of the results showed that the results of the analysis of the results showed that after 20 days of education, the results of the analysis of the results showed that after 20 days of education, the results of the analysis of the results showed that the results of the analysis of the results showed that after 20 days of education, the results of the analysis of the results showed that after 20 days of education, the results of the analysis of the results showed that after 20 days of education, the results of the analysis of the results were analyzed. The length of the tail fork of the EE group is the same as that of the normal group, and the body weight is the same as that of the same group. The growth is accompanied by the increase in plasticity, which is usually caused by the presence of plasticity in the EE group. On the other hand, 40 days later, the results show that the results are the same as the results, and the results show that after 40 days, the results show that the results are the same after 40 days.

项目成果

環境エンリッチメントを利用したサケ稚魚の飼育効果について

利用环境富集技术培育幼鲑鱼的效果

• 发表时间: 2022
• 作者: 越野陽介;藤原真;阿部嵩志;上本鈴華;工藤秀明
• 通讯作者: 工藤秀明