Post-transcriptional regulation of phytohormone signal transduction in Arabidopsis immunity

拟南芥免疫中植物激素信号转导的转录后调控

• 批准号: 404317946
• 负责人: Dr. Wolfgang Hoehenwarter
• 金额: --
• 依托单位: Leibniz-Institut für Pflanzenbiochemie (IPB)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/404317946?language=en
• 关键词: Post; transcriptional; regulation; phytohormone; signal

Post-transcriptional processes have recently been shown to play significant roles in adapting protein abundance in transitions from growth to immunity in plants. Phytohormones are known to govern most aspects of these steady states and their transitions. Here we propose a targeted multi-omics approach with a primary focus on targeted proteomics measurements of protein turn-over rates. We will investigate the chronology of transcriptional, post-transcriptional/translational and post-translational (protein degradation) processes of SA, JA, ET and auxin interaction in the establishment and transition of and between growth and immunity. We will also examine if altered degradation rates of abundant photosynthetic and primary metabolic enzymes are involved in remodeling the cellular proteome as a whole in the transition from growth to immunity. These questions are important to an understanding of the growth defense trade-off in plants.

在植物从生长到免疫的转变过程中，转录后过程在调节蛋白质丰度方面发挥了重要作用。众所周知，植物激素控制着这些稳定状态及其转变的大多数方面。在这里，我们提出了一种靶向多组学方法，主要关注蛋白质周转率的靶向蛋白质组学测量。我们将研究SA、JA、ET和生长素相互作用在生长和免疫的建立和转换过程中的转录、转录后/翻译和翻译后（蛋白质降解）过程的年表。我们还将研究在从生长到免疫的转变过程中，丰富的光合和初级代谢酶的降解率的改变是否参与了细胞蛋白质组的整体重塑。这些问题对于理解植物的生长防御权衡是很重要的。