Unrevealing the anti-obesity effects of wakame associated to sphingomyelin synthase inhibition

这项研究的目的是确定和调查海藻活性成分在肥胖与短信失衡相关的作用。初步的抑菌筛选结果表明，Wakame是潜在的候选菌株之一，当浓度为100μg/mL时，其抑制率分别为66%和75%。我在该项目中提出了以下目标：(1)对Wakame中的有效成分进行基于溶剂组分的筛选和鉴定(2)通过基于细胞的分析来检测该活性化合物对脂滴聚集和氧化的体外影响。按照所提出的方法，将蜡梅样品大规模收购，粉碎成粉，并制备了三次粗甲醇萃取物。由于WAKAME的甲醇提取物显示了良好的抗短信抑制作用，下一步是鉴定和研究WAKAME的活性成分的作用。此外，还进行了溶剂型隔板的制备。对正己烷、乙醚、乙醇、正丁醇、水基等溶剂进行了筛选，发现正己烷组分具有较强的缓蚀活性。我的目标是提纯活性化合物。与此同时，一旦化合物被提纯，其他与细胞相关的实验就开始了。这一转变将有助于开发以WAKAME为基础的营养食品来控制肥胖。

The purpose of this research is to identify and investigate the role of active constituents of seaweeds in obesity associated with SMS imbalance. Preliminary inhibition screening results showed Wakame as one of the potential candidates with 66% (SMS1) and 75% (SMS2) at 100 μg/mL. I have proposed the following objectives in the project(1) Perform solvent-fraction-based screening and identification of the active constituent in Wakame (2) Examine the in vitro effects of the active compound on lipid droplet accumulation and oxidation by cell-based assay. As proposed the wakame samples were purchased on a large scale and grind into powder and its crude methanol extract was prepared three times. As the methanolic extract of wakame showed a promising result against SMS inhibition, the next step is to identify and investigate the role of an active constituent of wakame. further, solvent-based partitions were made. Solvents such as Hexane, Ether, Ethanol, butanol, and water-based screening have been performed and found that hexane fraction has potent inhibition activity. I am aiming to purify the active compound. Meantime other cell-related experiments are started, as soon as the compound is purified. This turn will help the development of wakame-based nutraceuticals to control obesity.