Unrevealing the anti-obesity effects of wakame associated to sphingomyelin synthase inhibition

本研究的目的是确定和调查海藻的活性成分在与SMS失衡相关的肥胖中的作用。初步的抑制筛选结果显示，在100 μg/mL浓度下，裙带菜为66%（SMS 1）和75%（SMS 2）的潜在候选物之一。本人在本课题中提出了以下目标（1）对裙带菜中的活性成分进行基于溶剂级分的筛选和鉴定（2）通过基于细胞的测定来检查活性化合物对脂滴蓄积和氧化的体外作用。根据建议，大量购买裙带菜样品并研磨成粉末，并将其粗甲醇提取物制备三次。由于裙带菜的甲醇提取物显示出对抗SMS抑制的有希望的结果，下一步是鉴定和研究裙带菜的活性成分的作用。进一步进行溶剂基分配。已进行溶剂如己烷、乙醚、乙醇、丁醇和水基筛选，发现己烷馏分具有强抑制活性。我的目标是提纯活性化合物。同时，一旦化合物被纯化，就开始其他细胞相关的实验。这一转变将有助于开发以裙带菜为基础的营养品来控制肥胖。

The purpose of this research is to identify and investigate the role of active constituents of seaweeds in obesity associated with SMS imbalance. Preliminary inhibition screening results showed Wakame as one of the potential candidates with 66% (SMS1) and 75% (SMS2) at 100 μg/mL. I have proposed the following objectives in the project(1) Perform solvent-fraction-based screening and identification of the active constituent in Wakame (2) Examine the in vitro effects of the active compound on lipid droplet accumulation and oxidation by cell-based assay. As proposed the wakame samples were purchased on a large scale and grind into powder and its crude methanol extract was prepared three times. As the methanolic extract of wakame showed a promising result against SMS inhibition, the next step is to identify and investigate the role of an active constituent of wakame. further, solvent-based partitions were made. Solvents such as Hexane, Ether, Ethanol, butanol, and water-based screening have been performed and found that hexane fraction has potent inhibition activity. I am aiming to purify the active compound. Meantime other cell-related experiments are started, as soon as the compound is purified. This turn will help the development of wakame-based nutraceuticals to control obesity.