Unrevealing the anti-obesity effects of wakame associated to sphingomyelin synthase inhibition

揭示裙带菜与鞘磷脂合酶抑制相关的抗肥胖作用

• 批准号: 22K14850
• 负责人: DIVYAVANI
• 金额: $ 2.91万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K14850/
• 关键词: Wakame; Obesity; sphingolipid; LCMS

The purpose of this research is to identify and investigate the role of active constituents of seaweeds in obesity associated with SMS imbalance. Preliminary inhibition screening results showed Wakame as one of the potential candidates with 66% (SMS1) and 75% (SMS2) at 100 μg/mL. I have proposed the following objectives in the project(1) Perform solvent-fraction-based screening and identification of the active constituent in Wakame (2) Examine the in vitro effects of the active compound on lipid droplet accumulation and oxidation by cell-based assay. As proposed the wakame samples were purchased on a large scale and grind into powder and its crude methanol extract was prepared three times. As the methanolic extract of wakame showed a promising result against SMS inhibition, the next step is to identify and investigate the role of an active constituent of wakame. further, solvent-based partitions were made. Solvents such as Hexane, Ether, Ethanol, butanol, and water-based screening have been performed and found that hexane fraction has potent inhibition activity. I am aiming to purify the active compound. Meantime other cell-related experiments are started, as soon as the compound is purified. This turn will help the development of wakame-based nutraceuticals to control obesity.

本研究的目的是确定和研究海藻活性成分在与SMS失衡相关的肥胖中的作用。初步筛选结果显示，裙带菜在100 μg/mL时的抑制率分别为66% （SMS1）和75% （SMS2）。我在项目中提出了以下目标：(1)进行基于溶剂分馏的Wakame有效成分的筛选和鉴定(2)通过基于细胞的实验检查活性化合物对脂滴积累和氧化的体外影响。根据建议，大规模采购裙带菜样品，研磨成粉末，三次制备其粗甲醇提取物。由于裙带菜的甲醇提取物显示出良好的抗SMS抑制效果，下一步是鉴定和研究裙带菜的活性成分的作用。此外，还制作了溶剂型分区。溶剂如己烷、乙醚、乙醇、丁醇和水基筛选，发现己烷馏分有很强的抑制活性。我的目标是纯化活性化合物。同时，一旦化合物被纯化，其他与细胞相关的实验就会开始。这一转变将有助于开发以裙带菜为基础的营养保健品来控制肥胖。