Elucidating the role of IL-1R2, a decoy receptor of IL-1, in inflammation-induced stem cell aging

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Experimental Plan1: We successfully generated Il1r2CreER/Rosa-tdTomato lines. Tamoxifen-treated Il1r2CreER/Rosa-tdTomato mice, either through intraperitoneal injection (IP) or diet, exhibited tomato signal specifically in the inter-scale region, a typical territory of slow-cycling stem cells in the mouse tail skin.The lineage tracing of these tomato-positive basal cells in homeostasis or IL1a/b-induced condition will reveal their stem cell potential and behavior.Experimental Plan 2: Using the Tet-on system, we successfully generated an Il1r2 over-expressing mouse line. Il1r2 over-expressing mice counteracted the effect of TPA, an IL1a-mediated inflammatory agent, on tail skin compared to wild-type control mice.