Elucidating the role of IL-1R2, a decoy receptor of IL-1, in inflammation-induced stem cell aging

阐明 IL-1R2（IL-1 的诱饵受体）在炎症诱导的干细胞衰老中的作用

• 批准号: 22K15126
• 负责人: NGUYEN THI・KIM・NGUYEN
• 金额: $ 3万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K15126/
• 关键词: IL-1R2; TPA; Tet-on system; Epidermal Stem Cells; Il1r2; Chronic inflammation

Experimental Plan1: We successfully generated Il1r2CreER/Rosa-tdTomato lines. Tamoxifen-treated Il1r2CreER/Rosa-tdTomato mice, either through intraperitoneal injection (IP) or diet, exhibited tomato signal specifically in the inter-scale region, a typical territory of slow-cycling stem cells in the mouse tail skin.The lineage tracing of these tomato-positive basal cells in homeostasis or IL1a/b-induced condition will reveal their stem cell potential and behavior.Experimental Plan 2: Using the Tet-on system, we successfully generated an Il1r2 over-expressing mouse line. Il1r2 over-expressing mice counteracted the effect of TPA, an IL1a-mediated inflammatory agent, on tail skin compared to wild-type control mice.

实验计划1：成功生成Il1r2CreER/Rosa-tdTomato品系。他莫昔芬处理的Il1r2CreER/Rosa-tdTomato小鼠，无论是通过腹腔注射（IP）还是通过饮食，都在小鼠尾部皮肤的尺度间区域特异性地表现出番茄信号，这是一个典型的慢循环干细胞区域。这些番茄阳性基底细胞在稳态或IL1a/b诱导条件下的谱系追踪将揭示它们的干细胞潜能和行为。实验计划2：使用Tet-on系统，我们成功地产生了Il1r2过表达的小鼠系。与野生型对照小鼠相比，Il1r2过表达小鼠抵消了TPA（一种Il1r2介导的炎症剂）对尾皮的影响。