Elucidating the role of IL-1R2, a decoy receptor of IL-1, in inflammation-induced stem cell aging

实验计划1：我们成功地产生了Illr 2CreER/Rosa-tdTomato系。三苯氧胺处理的Il 1 r2 CreER/Rosa-tdTomato小鼠，无论是通过腹腔注射（IP）还是饮食，都在鳞片间区域（小鼠尾部皮肤中慢循环干细胞的典型区域）特异性地显示出番茄信号。这些番茄阳性基底细胞在稳态或IL 1a/b诱导条件下的谱系追踪将揭示它们的干细胞潜能和行为。实验方案2：使用Tet-on系统，我们成功地产生了IL 1 r2过表达小鼠系。 与野生型对照小鼠相比，IL 1 r2过表达小鼠抵消了TPA（一种IL 1a介导的炎症因子）对尾部皮肤的影响。

Experimental Plan1: We successfully generated Il1r2CreER/Rosa-tdTomato lines. Tamoxifen-treated Il1r2CreER/Rosa-tdTomato mice, either through intraperitoneal injection (IP) or diet, exhibited tomato signal specifically in the inter-scale region, a typical territory of slow-cycling stem cells in the mouse tail skin.The lineage tracing of these tomato-positive basal cells in homeostasis or IL1a/b-induced condition will reveal their stem cell potential and behavior.Experimental Plan 2: Using the Tet-on system, we successfully generated an Il1r2 over-expressing mouse line. Il1r2 over-expressing mice counteracted the effect of TPA, an IL1a-mediated inflammatory agent, on tail skin compared to wild-type control mice.