Creation and biological evaluation of nanoparticle-based artificial transcription factor

基于纳米颗粒的人工转录因子的创建和生物学评价

• 批准号: 22K15249
• 负责人: CHINNATHAMBI Shanmugavel
• 金额: $ 3万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K15249/
• 关键词: Transcription factor; Silica nanoparticles; Mitochondrial factors; Cell Imaging; Molecular modeling; medaka egg imaging; NANO-TF

Silicon quantum dots (SiQDs) capable of in vitro and in vivo imaging could dissect cellular function at the molecular level and have been advancing as a potent diagnostic tool in precision medicine. While numerous reports focus on evaluating the staining capacity of silica quantum dots, their nano-bio interaction, which is critical for assessing their biocompatibility, is often overlooked. We developed water-borne, NIR-emitting Pluronic F127-covered SiQDs micelles capable of live cell imaging with minimal cytotoxicity. The PLQY of the SiQDs/F127 micelles in water was 25% at pH 7. SiQD-De/F127 micelles demonstrated significant in vitro imaging capacity and limited cytotoxicity in the HeLa and human dermal fibroblasts. Furthermore, the SiQD-De/F127 micelles displayed excellent in vivo imaging capacity without requiring vectors and transfection agents when incubated with medaka fish eggs and larvae. It is important to note here that even at higher concentrations (1 mg mL－1), the normal embryonic development of medaka fish was not altered as the larvae hatched on day 17 from the micelles-treated egg as that of the control without any signs of toxicity. Therefore, SiQDs-De/F127 micelles are SiQDs expected to have excellent biocompatibility for in vivo imaging studies.

硅量子点（SiQDs）具有体外和体内成像能力，可以在分子水平上解剖细胞功能，并已成为精密医学中强有力的诊断工具。虽然许多报告都集中在评估二氧化硅量子点的染色能力，但它们的纳米生物相互作用是评估其生物相容性的关键，但往往被忽视。我们开发了水性，nir发射Pluronic f127覆盖的SiQDs胶束，能够以最小的细胞毒性进行活细胞成像。pH为7时，SiQDs/F127胶束在水中的PLQY为25%。SiQD-De/F127胶束在HeLa和人真皮成纤维细胞中表现出显著的体外成像能力和有限的细胞毒性。此外，SiQD-De/F127胶束在与medaka鱼卵和幼虫孵育时显示出良好的体内成像能力，无需载体和转染剂。值得注意的是，即使在更高的浓度下（1mg mL-1），也没有改变medaka鱼的正常胚胎发育，因为在第17天从胶束处理过的卵中孵化出的幼虫与对照组一样，没有任何毒性迹象。因此，SiQDs- de /F127胶束是有望在体内成像研究中具有良好生物相容性的SiQDs。

项目成果

A biophysical approach of cytarabine anticancer drug insights into human serum albumin and checkpoint kinase 1

阿糖胞苷抗癌药物的生物物理方法深入了解人血清白蛋白和检查点激酶 1

• DOI: 10.1016/j.rechem.2022.100755
• 发表时间: 2023
• 期刊: Results in Chemistry
• 影响因子: 2.3
• 作者: Rupavarshini Manoharan;Karthikeyan Subramani;Anandh Sundaramoorthy;Ramamoorthi Anitha;Ramakrishnamurthy Suganya;Bharanidharan Ganesan;Aruna Prakasarao;Mangaiyarkarasi Rajendiran;Chinnathambi Shanmugavel;Pandian Ganesh N.;Ganesan Singaravelu
• 通讯作者: Ganesan Singaravelu

Vellore Institute of Technology, Chennai(インド)

韦洛尔理工学院，金奈（印度）

Facile Synthesis of Hafnium Oxide Nanoparticle Decorated on Graphene Nanosheet and Its Photocatalytic Degradation of Organic Pollutants under UV-Light Irradiation

石墨烯纳米片修饰氧化铪纳米粒子的简便合成及其在紫外光照射下光催化降解有机污染物

• DOI: 10.3390/app122111222
• 发表时间: 2022
• 期刊: Applied Sciences
• 作者: Jayaraman Venkatachalam;Mahalingam Shanmugam;Chinnathambi Shanmugavel;Pandian Ganesh N.;Prakasarao Aruna;Ganesan Singaravelu;Ramasamy Jayavel;Ayyaru Sivasankaran;Ahn Young-Ho
• 通讯作者: Ahn Young-Ho

University of Science(マレーシア)

理科大学（马来西亚）

Multi-functional Transcription Therapeutics for a Mitochondrial Genetic Disorder

线粒体遗传性疾病的多功能转录疗法

• 发表时间: 2022
• 作者: Chinnathambi,S.;Lee,K.B.;Sugiyama,H.;Ganesh N Pandian
• 通讯作者: Ganesh N Pandian