Proof-of-concept Study for Podoplanin-targeted Radioimmunotherapy for Glioblastoma as a New Therapeutic Option

我们选择高表达PDPN的脑肿瘤细胞系LN319和低表达PDPN的脑肿瘤细胞系U87MG进行本课题的研究。由于U87MG是一种荧光素酶表达细胞系，因此我们可以将其用于原位脑肿瘤模型。我们用111In标记NZ16并进行细胞结合实验，发现U87MG显示低PDPN表达。作为前期研究，我们将U87MG-Luc细胞系接种于小鼠脑内，建立正交异性脑肿瘤模型，利用免疫荧光成像技术观察肿瘤随时间的生长情况。我们已经完成了脑肿瘤组织阵列的免疫组化，这是一个图像分析和计算的过程。

To conduct this research project, we choose high PDPN expressing brain tumor cell line LN319 and low PDPN expressing cell line U87MG. As U87MG is a luciferase expressing cell line, so we can use it for Orthotopic brain tumor model. We have labeled NZ16 with 111In and performed cell binding assay and found U87MG showed low PDPN expression. As a pilot study we inoculate U87MG-Luc cell line into mouse brain and create Orthotropic Brain tumor model and to see the tumor growth with time with immunofluorescence image using IVIS. We have done IHC of Brain tumor tissue array and this is on process of image analysis and calculation.