Proof-of-concept Study for Podoplanin-targeted Radioimmunotherapy for Glioblastoma as a New Therapeutic Option

为了进行本研究，我们选择了高表达PDPN的脑瘤细胞株LN319和低表达PDPN的细胞株U87 MG。由于U87 MG是一种表达荧光素酶的细胞系，因此我们可以将其用于原位脑瘤模型。我们用111In标记NZ16，并进行细胞结合实验，发现U87 MG显示低表达的PDPN。作为初步研究，我们将U87 MG-Luc细胞系接种到小鼠脑内，建立了正交异性脑瘤模型，并用IVIS免疫荧光图像观察肿瘤的生长情况。我们对脑肿瘤组织阵列进行了IHC，这是对图像进行分析和计算的过程。

To conduct this research project, we choose high PDPN expressing brain tumor cell line LN319 and low PDPN expressing cell line U87MG. As U87MG is a luciferase expressing cell line, so we can use it for Orthotopic brain tumor model. We have labeled NZ16 with 111In and performed cell binding assay and found U87MG showed low PDPN expression. As a pilot study we inoculate U87MG-Luc cell line into mouse brain and create Orthotropic Brain tumor model and to see the tumor growth with time with immunofluorescence image using IVIS. We have done IHC of Brain tumor tissue array and this is on process of image analysis and calculation.