Structure and mechanism of CRISPR-Cas type IV systems

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CRISPR-Cas systems are structurally and mechanistically highly diverse prokaryotic adaptive immune systems that protect against mobile genetic elements. Class I CRISPR-Cas systems feature multi-subunit effector complexes. We could recently demonstrate that even closely related subtypes, i.e. the type I-F and I-F variant (I-Fv), differ substantially in their structure and DNA surveillance mechanism. Furthermore, CRISPR-Cas systems have been implied to function in non-canonical processes, such as e.g. transcriptional control, stress response and pathogenicity development. Others and we could show that the CRISPR adaptation protein Cas1 interacts with the redox-stress sensing and signaling two-component system YedVW. The aim of this proposal is to produce an en-detail structural, mechanistic and functional understanding of poorly understood class I CRISPR-Cas systems, such as e.g. the Shewanella putrefaciens type I-Fv and the plasmid borne Aromatoleum aromaticum type IV system. Moreover, we aim at a structural, mechanistic and functional understanding of the interaction of Cas1 and YedVW in the redox-stress induced stress response of Escherichia coli.