Marburg core facility for Interaction, Dynamics and Assembly of biomolecular Structures

深入分析生物分子复合体的动力学和结构特征及其分子机制，不仅对现代生命科学至关重要，而且对于理解疾病和生物工程也是至关重要的。氢-氚交换质谱仪(HDX)和高通量结晶平台支持的X射线结晶学(HTP-X)是高度互补的、最先进的高分辨率动态和结构分析方法。随着德国一所大学于2015年安装了迄今为止第一条也是唯一一条自动HDX管道，我们经历了科学界对核心设施日益增长的需求(支持函证明了这一点)，在那里，两种技术将在一个标准化的设置中相互联系，并提供科学和行政支持。因此，我们提出了DFG核心设施马尔堡相互作用、动力学和生物分子组装结构(MIDAS)核心设施，集成在菲利普斯大学多学科和高可见度的洛韦合成微生物学中心(SYNMIKRO)。Midas将把其创新的自动化HDX管道与现有的HTP-X合并为一个独特的科学基础设施，可供广大国内和国际用户使用。在3年的资助期内，MIDAS将成为一个高度专业化和标准化的CF，具有明确的治理结构。这将通过在线用户手册中记录的用户界面、数据管理系统和预定义工作包(WPS)实现，并受附则(Nutzungsordnung)管理。将通过测试用户友好性(TUF)来持续监控CF的性能，包括其WPS。此外，Midas将通过其年度用户会议和2018年关于HDX和结构方法的国际暑期学校来促进交流和教育。总而言之，随着拟议的技术、科学和行政支持的安装，MIDAS将成为DFG的一个引人注目的核心设施；模拟如何将最先进的技术经济地合并到优秀的功能单位中，作为协作研究倡议、中心和卓越集群的核心单位。

In-depth analysis of the dynamic and structural features of biomolecular complexes and their molecular mechanisms is crucial for the modern life sciences, but also understanding disease and bioengineering. Hydrogen-Deuterium eXchange mass spectrometry (HDX) and X-ray crystallography empowered by high-throughput crystallization platforms (HTP-X) are highly complementary, state-of-the-art methods for the dynamic and structural analysis at high resolution. With the installation of the up to now first and only automated HDX pipeline at a German University in 2015, we experienced an increasing need of the scientific community for a core facility (as evidenced by the Support letters), where both technologies would be interlinked in a standardized setup with scientific and administrative support. Therefore, we propose the DFG core facility Marburg core facility for Interactions, Dynamics and biomolecular Assembly Structure (MIDAS) integrated in the multidisciplinary and highly visible LOEWE Center of Synthetic Microbiology (SYNMIKRO) of the Philipps-University. MIDAS will merge its innovative automated HDX pipeline with established HTP-X into a unique scientific infrastructure available to a broad national and international user community. In the 3-year funding period, MIDAS will become a highly professionalized and standardized CF with a defined governance structure. This will be enabled by a user interface, data management system and predefined work packages (WPs) documented in an Online User Manual and regulated by bylaw (Nutzungsordnung). Performance of the CF including its WPs will be continuously monitored through Testing User Friendlyness (TUF). Further, MIDAS will promote communication and education through its annual user meetings and an international summerschool on HDX and structural methods in 2018. Taken together, with the installation of the proposed technical, scientific and administrative support, MIDAS will become a highly visible DFG core facility; modeling how the state-of-the-art technology can be economically merged into excellent functional units serving as core units within collaborative research initiatives, centers and excellence clusters.