How genetic abnormality in keratinocytes, not in melanocytes, causes pigmentary skin disorders?

角质形成细胞而非黑素细胞的遗传异常如何导致色素性皮肤病？

• 批准号: 22K16289
• 负责人: ホセイン エムディ・ラジブ
• 金额: $ 3万
• 依托单位: Jichi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K16289/
• 关键词: pigmentation; keratinocyte; genetic abnormality; keratin 5; melanosome

Isolate the K5 mutant (P25L) gene: The K5 mutant gene with the specific mutation (P25L) is obtained by introducing the desired mutation using molecular biology techniques and Cloning the K5 mutant gene into adenovirus victor. generate Crispr-Cas-based knock-in mice with an inducible mutant K5 we have designed guide RNA (gRNA): A specific gRNA is designed to target the desired genomic location where the knock-in will occur.

K5突变体（P25 L）基因的分离：利用分子生物学技术将所需突变导入腺病毒载体维克托，获得具有特异性突变（P25 L）的K5突变体基因。我们设计了指导RNA（gRNA）：设计特异性gRNA以靶向将发生敲入的所需基因组位置。