Tissue diagnosis using software-supported assessment of biomarkers

使用软件支持的生物标志物评估进行组织诊断

• 批准号: 405888926
• 负责人: Dr. Christian Ostalecki
• 金额: --
• 依托单位: Hautklinik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/405888926?language=en
• 关键词: Tissue; diagnosis; using; software; supported

The overall goal of our research is to analyze the molecular mechanisms leading to the development of cutaneous melanoma, in order to develop biomarker profiles and innovative therapeutic approaches. All these aspects were included in this proposal.Our previous results revealed that activated ADAM10 may play an important role in many forms of cutaneous melanoma, based on the analysis of signaling pathways in melanoma cell lines in vitro. In a recently published study we could verify this finding in melanoma tissue and describe the mechanism of ADAM10 activation. We discovered that the endopeptidase SPPL3 acted as a crucial upstream activator of ADAM10. Using the so-called MELC (multi epitope ligand cartography) technology was crucial in this approach. This technology allows the assessment of more than 100 different antigens on one tissue/cell layer and within their topographical localization. In this proposal we aim to analyze whether our findings and biomarkers could be applied to other forms of cutaneous melanoma and if the identified tissue biomarker profiles could be used for tissue diagnosis by means of the MELC technology, powered by an image analysis software. Furthermore, the role of the early tumor microenviroment in ADAM10 activation will be investigated.

我们研究的总体目标是分析导致皮肤黑色素瘤发展的分子机制，以开发生物标志物和创新的治疗方法。本研究通过对体外培养的黑色素瘤细胞系的信号转导通路的分析，揭示了活化的ADAM 10在多种皮肤黑色素瘤中可能发挥重要作用。在最近发表的一项研究中，我们可以在黑色素瘤组织中验证这一发现，并描述ADAM 10激活的机制。我们发现，内肽酶SPPL 3作为一个重要的上游激活剂的ADAM 10。使用所谓的MELC（多表位配体制图）技术在这种方法中至关重要。该技术允许在一个组织/细胞层上和在它们的地形定位内评估超过100种不同的抗原。在这项提案中，我们的目标是分析我们的研究结果和生物标志物是否可以应用于其他形式的皮肤黑色素瘤，以及所确定的组织生物标志物谱是否可以通过由图像分析软件提供动力的MELC技术用于组织诊断。此外，还将研究早期肿瘤微环境在ADAM 10激活中的作用。