Cell morphogenesis by the subcellular spatial segregation of molecular mobility on biomaterial surface

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In the current study, the applicant （1）successfully fabricated the micropatterned subcellular spatial extracellular matrix (ECM) strips by microcasting method with 1% of DMS-co-EO containing PDMS mold. （2) observation of ECM(collagen) gel strips.（3）the fibroblast cell morphology onto the micropatterned subcellular spatial ECM (collagen) strips was observed. The fibroblast cell elongated its morphology on the collagen strips..The detailed contents are listed in the following. (1) The applicant has successfully designed a unique substrate with the subcellularsized spatial stripes of collagen ECM strips， It is expected to have different surface mobility. (2) After the collagen strips were fabricated, the applicant did the observation of collagen gel strips by several methods, including florescent staining of collagen gel strips , AFM observation of the 3D topography of collagen gel strips, second-harmonic generation (SHG) observation of fiber structure in the collagen gel strips. (3) Evaluation of the cell morphogenesis on biomaterial surfaces with micropatterned subcellular spatial ECM strips. Cell attachment area and aspect ratio (the ratio of the short axis divided by the long axis of the best-fit ellipse) was assessed.The significance and importance of the study are that the applicant successfully fabricated substrate with micropatterned ECM strips based on micro-casting. It pointed out the importance of the ECM stirp on the cell morphogenesis, which can work as a fundamental basement for further study of surface mobility.