Cell morphogenesis by the subcellular spatial segregation of molecular mobility on biomaterial surface

生物材料表面分子运动的亚细胞空间分离导致的细胞形态发生

• 批准号: 22K18200
• 负责人: Han Aifang
• 金额: $ 2.91万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K18200/
• 关键词: cell morphogenesis; biomaterial; extracellular matrix; micro-casting; surface mobility

In the current study, the applicant （1）successfully fabricated the micropatterned subcellular spatial extracellular matrix (ECM) strips by microcasting method with 1% of DMS-co-EO containing PDMS mold. （2) observation of ECM(collagen) gel strips.（3）the fibroblast cell morphology onto the micropatterned subcellular spatial ECM (collagen) strips was observed. The fibroblast cell elongated its morphology on the collagen strips..The detailed contents are listed in the following. (1) The applicant has successfully designed a unique substrate with the subcellularsized spatial stripes of collagen ECM strips， It is expected to have different surface mobility. (2) After the collagen strips were fabricated, the applicant did the observation of collagen gel strips by several methods, including florescent staining of collagen gel strips , AFM observation of the 3D topography of collagen gel strips, second-harmonic generation (SHG) observation of fiber structure in the collagen gel strips. (3) Evaluation of the cell morphogenesis on biomaterial surfaces with micropatterned subcellular spatial ECM strips. Cell attachment area and aspect ratio (the ratio of the short axis divided by the long axis of the best-fit ellipse) was assessed.The significance and importance of the study are that the applicant successfully fabricated substrate with micropatterned ECM strips based on micro-casting. It pointed out the importance of the ECM stirp on the cell morphogenesis, which can work as a fundamental basement for further study of surface mobility.

在本研究中，申请人(1)以1%的含PDMS模具的DMS-co-EO为原料，采用微铸法制备了微图案化的亚细胞空间细胞外基质(ECM)条带。(2)细胞外基质(ECM)凝胶条的观察。(3)成纤维细胞在微图案化的亚细胞空间ECM(胶原)条上的细胞形态观察。成纤维细胞在胶原条上拉长其形态。具体内容如下。(1)申请人成功地设计了一种独特的基板，具有亚细胞尺寸的胶原ECM条的空间条纹，预计具有不同的表面迁移率。(2)胶原条制作完成后，申请人用多种方法对胶原胶原胶条进行观察，包括胶原胶原胶条的荧光染色、胶原胶原胶条三维形貌的AFM观察、胶原胶原胶条内纤维结构的二次谐波(SHG)观察。(3)用微图案化的亚细胞空间ECM条评价生物材料表面的细胞形态发生。细胞附着面积和长宽比(最适合椭圆的短轴除以长轴的比率)被评估。本研究的意义和重要性在于申请人成功地基于微铸造制造了具有微图案化ECM条的基板。指出了ECM STRIP对细胞形态发生的重要性，为进一步研究表面迁移率奠定了基础。

项目成果

Microcast of 3D collagen gel stripes can regulate cell morphology

3D 胶原凝胶条纹的微铸可以调节细胞形态

• 发表时间: 2022
• 作者: Yasunobu Yamashita ;Hirofumi Yamazaki ;Toshio Shimokawa ;Yuki Kawaji ;Takashi Tamumra ;Keiichi Hatamaru ;Masahiro Itonaga ;Reiko Ashida; Masayuki Kitano;Aifang Han;Aifang Han
• 通讯作者: Aifang Han

Spatial control of cell microenvironment through micro-casting of 3D collagen strips

通过 3D 胶原条的微铸造来空间控制细胞微环境

• 发表时间: 2022
• 作者: Yasunobu Yamashita ;Hirofumi Yamazaki ;Toshio Shimokawa ;Yuki Kawaji ;Takashi Tamumra ;Keiichi Hatamaru ;Masahiro Itonaga ;Reiko Ashida; Masayuki Kitano;Aifang Han;Aifang Han;Aifang Han
• 通讯作者: Aifang Han

Effect of dimethylsiloxane-co-ethylene oxide block copolymer addition into polydimethylsiloxane on the harvesting of collagen micropattern by microcasting

聚二甲基硅氧烷中添加二甲基硅氧烷-环氧乙烷嵌段共聚物对微铸造收获胶原微图案的影响

• 发表时间: 2022
• 作者: Aifang Han;Nobuyuki Tanaka;Koki Yamamoto;Yo Tanaka
• 通讯作者: Yo Tanaka