Developmental Study on a Simultaneous Processing System for Microphotometry and Video Image of Dynamic Cell Function.

我们试图建立一个系统，同时进行视频成像和细胞功能的显微光度记录。该系统由一个光学系统和两个检测系统组成。在光学部分，我们使用了尼康显微镜（FLUOPHOT microphoto V系列）。在该显微镜中，通过放置在舞台上的制备物的显微物镜的光被半反射镜分成两个方向，两个方向的光被高信噪比的摄像机（Hamamatsu Phodonics,c-1800）和5 × 5元光电二极管阵列（Centronix, MD-25, Croydon，UK）检测。摄像机的输出输入图像处理器（Hamamatsu Photonics, C-1901），处理后的图像被传送到计算机系统并显示。计算机程序是用Pascal语言编写的。另一方面，光电二极管阵列的输出被传递给16个放大器、一个多路复用器和一个a /D转换器，然后它们被传送到计算机并显示。我们将该系统应用于监测蛙心房制剂的自发电兴奋和耦合收缩。利用光电二极管阵列和电压敏感染料对电激励相关光信号进行检测，获得了接触相关光散射变化的拓扑图像。此外，利用该系统，通过计算机辅助数据处理，成功地分离了激励信号和收缩信号。

We have tried to ccqnststruct a system to simultaneously carry out both the video-imaging and the microphotometric recording of cell functions. The system is composed of an optics and two detection systems.In the part of optics, we used a Nikon microscope (FLUOPHOT microphoto V Series). In this microscope, light via a microscopic objective from preparation placed on the stage was devided in two directions with a half mirror, and the two devided light were detected by a high S/N ratio video camera (Hamamatsu Phodonics,c-1800) and a 5 x 5-element photodiode array (Centronix, MD-25, Croydon,UK) in each direction. An output from the video camera fed into an image processof (Hamamatsu Photonics, C-1901), and the processed image was transfered to a computer system and displayed. The computer programs were written in Pascal language. On the ogher hand, the outputs from the photodiode array were passed to 16- amplifiers, a multiplexer and an A/D converter, and then they were transfered to the computer and displayed.We have applied this system to monitor the spontaneous electrical excitation and coupled contraction of the frog atrium preparations. The electrical excitation-related optical signals were detected using the photodiode array together with voltage-sensitive dyes, and the toporogical images of the contnaction related light scattering changes were obtained. Furthermore, using this system, we succeeded to separate the excitation-signal and contraction-signal by computer-aided data processing.