Development of Rapid Masspropagation System for Tissue-cultured Plantlets.

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We developed a prototype of photoautotrophic plant micropropagation system. With this system, environmental factors such as temperature, humibity, light intensity and PH of nutrient solution can be controlled. We conducted a series of test run and proved that the environment can be controlled with desired ranges.A series of experiments was conducted under different envirironmental conditions in order to find favorable environmental conditions for promoting the growth of plantlets in vitro at multiplication and rooting stages. Carnations, strawberries, potato etc. were used as plant materials.Then, we found that explants with a small leaf containing chlorophyll can be differentiated and grown photoautotrophically with sugar-free medium and that the growth is much promoted in photoautotrophic culture than in heterotrophic culture.Photoautotrophic micropropatation may have essential advantages against conventional heterotrophic micropropagation. Namely, a loss of plantlets due to contgamination can be reduced; a larger vessel in size can be used, automation of the micropropagation process can be realized, ctc.Energy analysis, growth analysis and matter production balance analysis were conducted in order to obtain the basic knowledge on the development of automated and energy- and labor saving photoautotrophic masspropagation system for tissue-cultured plantlets.Photosynthetic characteristics of tissue-cultured plantlets were measured for strawberry, potato and carnation. We found that their characteristics were basically similar to those for the plants grown exvitro.Based on the experimental results obtained through the present research we conclude that the photoautotrophic masspropagation is feasible on a commercial scale in the near future.