Development of Rapid Masspropagation System for Tissue-cultured Plantlets.

我们开发了一个光合自养植物微繁殖系统的原型。该系统可对温度、湿度、光照强度、营养液pH等环境因子进行控制。通过一系列的试验，证明环境可以控制在理想的范围内，并在不同的环境条件下进行了一系列的试验，以寻找促进试管苗增殖和生根阶段生长的有利环境条件。以香石竹、草莓、马铃薯等为材料，在无糖培养基上，发现具有含叶绿素的小叶片的外植体能在光自养条件下分化和生长，且光自养培养比异养培养对生长的促进作用更大，说明光自养微繁技术可能具有传统的异养微繁技术所不具备的优势。即可以减少由于污染造成的幼苗损失;可以使用尺寸更大的容器，可以实现微繁殖过程的自动化，ctc.能量分析，通过生长分析和物质生产平衡分析，为建立自动化、节能省工的组培苗光自养繁殖系统提供了基础知识。测量草莓、马铃薯和康乃馨的培养小植株。结果表明，它们的特性与离体培养的植株基本相似，根据本研究所获得的实验结果，我们认为，在不久的将来，光自养大规模繁殖是可行的。

We developed a prototype of photoautotrophic plant micropropagation system. With this system, environmental factors such as temperature, humibity, light intensity and PH of nutrient solution can be controlled. We conducted a series of test run and proved that the environment can be controlled with desired ranges.A series of experiments was conducted under different envirironmental conditions in order to find favorable environmental conditions for promoting the growth of plantlets in vitro at multiplication and rooting stages. Carnations, strawberries, potato etc. were used as plant materials.Then, we found that explants with a small leaf containing chlorophyll can be differentiated and grown photoautotrophically with sugar-free medium and that the growth is much promoted in photoautotrophic culture than in heterotrophic culture.Photoautotrophic micropropatation may have essential advantages against conventional heterotrophic micropropagation. Namely, a loss of plantlets due to contgamination can be reduced; a larger vessel in size can be used, automation of the micropropagation process can be realized, ctc.Energy analysis, growth analysis and matter production balance analysis were conducted in order to obtain the basic knowledge on the development of automated and energy- and labor saving photoautotrophic masspropagation system for tissue-cultured plantlets.Photosynthetic characteristics of tissue-cultured plantlets were measured for strawberry, potato and carnation. We found that their characteristics were basically similar to those for the plants grown exvitro.Based on the experimental results obtained through the present research we conclude that the photoautotrophic masspropagation is feasible on a commercial scale in the near future.