Basic Research on Relationship between physical environment and growth of plantlets in vitro

自然环境与试管苗生长关系的基础研究

• 批准号: 02454088
• 负责人: KOZAI Toyoki
• 金额: $ 4.35万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454088/
• 关键词: Plant Tissue Culture; Micropropagation; Lighting System; Environmental Control; Photosynthesis; Optical Fibers; Tissue-cultured transplants; Physical Environment; 湿度; 蒸散特性; 培養

A novel lighting system for promoting the photosynthesis and growth of in vitro, plantlets with minimum electricity consumption was developed. The system is composed of a metal halide lamp, a refrector, a thermal filter, diffusive optical fibers, and culture vessels. Performance of the system was tested and evaluated for improving it further for practical application.With this system, the light source was placed outside the culture room. The light was focused at the edge of bunched fibers and sent to the culture room through diffusive optical fibers. With this system only photosynthetically active radiation is sent through the fibers. The fibers were placed adjacent to the side walls of vessels. Thus, the plantlets in vitro receive the light from the sides.Single node cuttings each with a small leaf were cultured in vitro with this system and the growth was compared with that of plantlets cultured in conventional a method. It was found that, the net photosynthesis, fresh weight, and dry weight were greater in the proposed method than in the conventional method. The plantlets cultured with this system were more vigorous and shorter than those cultured in the conventional way.With this system, cooling cost can be reduced, air space is more efficiently used, especially when culture vessels were stacked. Application of this system on a larger scale seems to be practical.

开发了一种以最小耗电量促进试管苗光合作用和生长的新型照明系统。该系统由金属卤化物灯、反射器、热过滤器、扩散光纤和培养容器组成。对该系统的性能进行了测试和评价，为进一步改进该系统的实际应用奠定了基础。将光聚焦在成束光纤的边缘，并通过漫射光纤发送到培养室。在这种系统中，只有光合有效辐射通过纤维传送。将纤维放置在血管侧壁附近。用该系统对带小叶的单节插条进行了离体培养，并与常规方法培养的试管苗进行了比较。结果表明，该方法的净光合速率、鲜重和干重均大于常规方法。该系统培养的试管苗比常规培养的试管苗生长健壮，植株矮小，降低了冷却成本，有效地利用了空气空间，尤其是在培养容器堆叠的情况下。在更大范围内应用该系统似乎是可行的。

项目成果

T.Kozai,N.Ohde and C.Kubota: "Similarity of Growth Between Plantlets and Seedlings of Brassica Campestris L.under Different in vitro Environmental Conditions" Plant Cell Tissue and Organ Culture. 24(3). 181-186 (1991)

T.Kozai，N.Ohde 和 C.Kubota：“不同体外环境条件下甘蓝幼苗和幼苗生长的相似性”植物细胞组织和器官培养。

T. Kozai (Ed. I. Karube): "Automation in Biotechnology (Environmental Control and Automation in Micropropagation)" Elsevier. 279-304 (1991)

T. Kozai（Ed. I. Karube）：“生物技术自动化（微繁殖中的环境控制和自动化）”Elsevier。

T.Kozai: "Photoautotrophic Micropropagation of <Rosa>___ー Plantlets Under High CO_2 and High photosynthetic Photon Flux Conditions" International Horticultural Congress XXIII (Firenze). 173 (1990)

T. Kozai：“<Rosa>___－在高 CO_2 和高光合光子通量条件下的植物的光自养微繁殖”国际园艺大会 XXIII（佛罗伦萨）173（1990）。

T.Kozai,K.Iwabuchi,K.Watanabe and I.Watanabe: "Photoautotrophic and Photomixotrophic Growth of Strawberry Plantlets in vitro and Changes in Nutrient Composition of the Medium" Plant Cell Tissue and Organ Culture. 25(2). 107-115 (1991)

T.Kozai、K.Iwabuchi、K.Watanabe 和 I.Watanabe：“草莓植株体外光合自养和光合营养生长以及培养基营养成分的变化”植物细胞组织和器官培养。

古在 豊樹,岩浪 好恵,渡部 桐絵: "ミクロ繁殖における環境調節と自動化" BIO INDUSTRY. 8(3). 28-35 (1991)

Toyuki Kozai、Yoshie Iwanami、Kirie Watanabe：“微型繁殖中的环境控制和自动化”BIO INDUSTRY 8(3) (1991)。