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Study on Environmental Control for Prevention against Sudden Population Decrease in Mass Production of the Rotifer Brachionus plicatilis.

防止褶皱臂尾轮虫大量生产中种群数量骤减的环境控制研究。

基本信息

批准号：
62860024
负责人：
HIRAYAMA Kazutsugu
金额：
$ 11.78万
依托单位：
FACULTY OF FISHERIES, NAGASAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

项目摘要

The rotifer Brachionus plicatilis was found to have requirements for vitamin C and cholesterol when evaluated using basic food suspension composed of baker's yeast and other essential vitamins. Two strains of marine yeasts were found to have higher food value for the rotifer than baker's yeast. Freshwater Chlorella enriched with vitamin B_<12> showed the food value which could match that of marine Chlorella. Stable mass production of the rotifer was achieved by the supply of the freshwater Chlorella enriched with vitamin B_<12>.Bacteria in a rotifer pond are proved to be important as an energy source and a supplier of essential vitamins. On the contrary, the venomous bacteria were found in mass culture pond of the rotifer which introduce sudden mass mortality. The rotifer culture was improved to attain extremely high density when bacterial flora was controlled by adding some useful bacteria periodically to the pond.Most of ciliated protozoa occurred in the culture water of the rotifer were identified as Uronema and Euplotes. These ciliates nurtured the certain bacterial flora, which repressed the growth of the rotifer. These ciliates could be removed by installing the substratum in the culture water of the rotifer where the protozoa tended to attach. Using Artemia at the growth stage of more than the 10th instar, protizoa were also removed by its feeding. The growth of flagellated protozoa which seriously fed on marine Chlorella could be repressed by adding NaCl at the final concentration more than 3 % in water.

轮虫褶皱臂尾轮虫被发现有维生素C和胆固醇的需求时，使用基本的食物悬浮液面包酵母和其他必需维生素组成的评估。两株海洋酵母对轮虫的食用价值高于面包酵母。富含维生素B_的淡水小球藻<12>具有与海水小球藻相当的食用价值。通过补充富含维生素B_的淡水小球藻，实现了轮虫的稳定批量生产<12>。轮虫池中的细菌被证明是重要的能量来源和必需维生素的供应者。相反，在轮虫的大量培养池中发现了有毒细菌，导致轮虫的突然大量死亡。通过定期添加有益菌控制水体中细菌植物群，使轮虫培养达到极高密度，轮虫培养水体中的纤毛虫主要为尾丝藻和游仆虫。这些纤毛虫滋养了特定的细菌植物群，抑制了轮虫的生长。在轮虫的养殖水体中原生动物容易附着的地方安装基质可以去除这些纤毛虫。在10龄以上的卤虫生长期，原生动物也被其摄食去除。当海水中NaCl浓度超过3%时，可抑制严重摄食海洋小球藻的鞭毛虫的生长。