权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Genetic Study on Variation among Strains of the Rotifer Brachionus plicatilis.

褶皱臂尾轮虫品系变异的遗传研究。

基本信息

批准号：
63560193
负责人：
HIRAYAMA Kazutsugu
金额：
$ 1.54万
依托单位：
FACULTY OF FISHERIES, NAGASAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63560193/
关键词：
Food organisms Brachionus plicatilis Isozyme Morphological variation Genetic variation Seedling 飼料生物 L型・S型

项目摘要

We collected 67 rotifer strains ( Brachionus plicatilis ) from many localities all over the world, including Japan. At first, we divided these strains into two types (L,30; S,37) by judging morphologically with the shape difference of anterior spines in lorica. Then, for each strain, 20 individuals which multiplied from one individual were measured morphometrically in 7 body parts and 5 varieties, when they grew up until laying the first eggs. The cluster analysis indicated that 67 strains can be divided into two large clusters, each of which consist only of group of L or S strains.Isozyme variations of 67 strains were detected by horizontal starch gel electrophoresis with a little amount of drip thawed from frozen stock as a crude enzyme. Six loci on 6 enzymes were interpretable as isozyme. Large differences in isozyme variation on 23 allele on 6 loci were recognized between L and S types. From genetic distances according to gene frequencies of 46 allele, the dendrogram among 67 strains was drawn. The strains were divided into two groups, each of which consists only of S type or L type strains. This result indicates the great genetical differences between L and S type strains.Among 37 S type strains, four strains showed isozyme patterns different from other S type strains. In these strains, three loci were particular for isozyme patterns. Morphometric studies showed that lorica sizes were much larger than the mean of 37 S type strains. The four strains might be triploid.

我们从包括日本在内的世界各地收集了67个轮虫菌株(褶皱臂尾轮虫)。首先，根据洛里卡前棘的形态差异，将这些菌株分为两型(L，30岁；S，37岁)。然后，对于每个品系，从一个个体繁殖出来的20个个体在7个身体部位和5个品种上进行了形态计量测量，从它们长大到产下第一个卵。聚类分析表明，67株菌株可分为两大类群，每一大类群仅由L菌株和S菌株组成，采用水平淀粉凝胶电泳法检测67株菌株的同工酶变异。6个酶上的6个基因座可解释为同工酶。在6个座位上的23个等位基因的同工酶变异在L和S两种类型之间存在较大差异。根据46个等位基因频率的遗传距离绘制了67个菌株的系统聚类图。菌株分为两组，每组仅由S和L两种类型的菌株组成。这说明L株和S株在遗传上存在较大差异，在37株S株中，有4株表现出与其他S株不同的同工酶图谱。在这些菌株中，有3个基因座具有特有的同工酶图谱。形态计量学研究表明，洛里卡菌株的大小远远大于37个S类型菌株的平均值。这四个菌株可能是三倍体。