Studies on the control of environmental bacterial flora for mass production of food microalgae

食品微藻大规模生产的环境菌群控制研究

• 批准号: 06660243
• 负责人: HIRAYAMA Kazutsugu
• 金额: $ 1.34万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660243/
• 关键词: Coexistence bacteria; Diatom Chaetoceros gracilis; Nannochloropsis oculata; Growth promoting factor; Biocontrol; Larval culture; Food organisms; 珪藻 Chaetoceros grasilis

1) Growth promoting effects of bacterial coexistence on marine diatom Chaetoceros gracilisWhen each of 12 bacterial strains isolated from diatom mass culture tanks, was added into the axnic culture of the diatom, Chaetoceras gracilis, only one strain, Fravobacterium sp.greatly promoted the diatom growth. In practical mass cultures of three marine microalgae (C.gracilis, Isochrysis galbana and Pavlova Iutheri) using 5 l flasks in indoor culture system, this bacterial strain was inoculated together with the microalgae at the inoculation. The maximum cell density of macroalgae was significantly higher than that in the control culture, and the stationary growth phase lasted longer than that of control.2) Growth-suppressing effect of the contaminated substances in one lot of NaCl special grade reagent on the population growth of so-called marine Chlorella and the elimination of the effect by the coexistence of bacterial strain.I cultured so-called marine Chlorella Nannochloropsis oculata in ASP6 medium prepared by some lots of NaCl special grade reagents, and then found one lot which did not support the algal growth. This contaminated substances were made clear as the organic substnces having less than 500 molecular weight. If the alga was cultured together with the bacterial strain Fravobacterium sp., it grew steadily as well as in ASP6 prepared by another lot Nacl. This fact tells us that this bacterial strains has the special ability for adsorption or absorption of these contaminated substances.

1)细菌共存对海洋硅藻Chaetoceros gracilis的促生长作用当从硅藻大量培养槽中分离的12株细菌分别加入到硅藻Chaetoceros gracilis的培养液中时，只有一株细菌Fravobacterium sp.对硅藻的生长有明显的促进作用。在室内培养系统中，使用5升烧瓶大量培养三种海洋微藻（C.gracilis，Isochrysis galbana和Pavlova Iutheri），在接种时将该菌株与微藻一起接种。大型藻类的最大细胞密度显著高于对照培养物，2）研究了一批NaCl特级试剂中污染物质对海洋小球藻种群生长的抑制作用及细菌菌株的共存对抑制作用的消除。在用几批NaCl特级试剂配制的ASP 6培养基中培养了一批海洋小球藻（Chlorella Nannochloropsis oculata），发现一批不支持藻类生长。这些污染物被明确为分子量小于500的有机物。如果将该细菌与细菌菌株Fravobacterium sp.一起培养，在另一批NaCl制备的ASP6中生长稳定。这一事实告诉我们，这种细菌菌株对这些污染物质具有特殊的吸附或吸收能力。

项目成果

A.Hagiwara,Min-Min Jung,T.Sato,K.Hirayama: "Interspecific Relations between Marine Rotifer Brachionus rotundiformis and Zooplankton Species Contaminating in the rotifer mass culture Tank" Fisheries Science. 61. 623-625 (1995)

A.Hagiwara、Min-Min Jung、T.Sato、K.Hirayama：“轮虫群体培养池中海洋轮虫 Brachionus rotundiformis 与浮游动物物种污染之间的种间关系”渔业科学。

A.Hgiwara, Min-Min Jung, T.Sato, K.Hirayama: "Interspecific Relations between Marine Rotifer Brachionus rotundiformis and Zooplankton Species Contaminating in the rotifer mass culture Tank." Fisheries Science. 61. 623-625 (1995)

A.Hgiwara、Min-Min Jung、T.Sato、K.Hirayama：“轮虫群体培养池中海洋轮虫 Brachionus rotundiformis 与浮游动物物种污染之间的种间关系。”

Suminto and Kazutsugu Hirayama: "Effects of Bacterial Coexiatence on the Growth of a Marine Diatom Chaetoceros gracilis" Fisheries Science. 62. 40-43 (1996)

Suminto 和 Kazutsugu Hirayyama：“细菌共存对海洋硅藻 gracilis 生长的影响”渔业科学。

Suminto and Kazutsugu Hirayama: "The practical use of a diatom growth-promoting bacteia for stable mass vulture of three marine maicroalgae" B.of Fac.Fish., Nagasaki Univ.(special issue). (in preparation).

Suminto 和平山 Kazutsugu：“硅藻生长促进细菌在稳定三种海洋微藻群体中的实际应用”B.of Fac.Fish.，长崎大学（特刊）。

Suminto and Kazutsugu Hirayama: "The practical use of a diatom growth-promoting bacteria for stable mass culture of three marine microalgae" 長崎大学水産学部研究報告. 特別号(予定). (1996)

Suminto 和 Kazutsugu Hirayyama：“硅藻生长促进细菌在三种海洋微藻的稳定大量培养中的实际应用”，长崎大学水产学院特刊（计划）。