Cellular and molecular characterization of a mouse model for WNT1-related autosomal recessive osteogenesis imperfecta

WNT1 相关常染色体隐性成骨不全小鼠模型的细胞和分子特征

• 批准号: 408075642
• 负责人: Dr. Timur Yorgan
• 金额: --
• 依托单位: Institut für Osteologie und Biomechanik (IOBM)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/408075642?language=en
• 关键词: Cellular; molecular; characterization; mouse; model

The Wnt signaling pathway is emerging as one of the most important regulatory mechanisms controlling bone remodeling. Although it is still unclear, which of the 19 known ligands acts as the primary osteoanablic molecule, there is increasing evidence that Wnt1 is one of the key players in the context of bone formation. In fact, whereas heterozygous WNT1 mutations were identified in patients with early-onset osteoporosis, homozygous WNT1 mutations cause severe childhood osteoporosis, classified as osteogenesis imperfecta type XV. Data generated by the IOBM support the relevance of Wnt1 in bone metabolism, since a mouse model with inducible Wnt1 expression in osteoblasts displayed a marked increase in bone mass due to increased osteoblast activity. Similarly, we observed reduced bone mass and increased fracture risk in a mouse model with osteoblast-specific Wnt1 deletion, whereas a mouse model of Wnt1-dependent early-onset osteoporosis displayed a significant reduction of trabecular and cortical bone mass.The present application aims at characterizing a mouse model carrying a specific mutation (G177C) of Wnt1, which was previously identified in a patient with osteogenesis imperfecta type XV. Initial results indicate that these animals do not only display reduced trabecular and cortical bone mass, but also a markedly increased fracture risk. In this proposal it will be addressed, (i) if the phenotype is primarily caused by an impaired quality of the bone matrix or by altered bone remodeling, (ii) which downstream pathways are mediating the osteoanabolic effect of Wnt1, and (iii) if teriparatide or anti-resorptive treatment will positively influence the phenotype. The expected results could not only optimize the treatment of patients with WNT1 mutations, but also be a basis for development of novel therapeutic options to treat different skeletal disorders.

Wnt信号通路是骨重建的重要调控机制之一。尽管目前还不清楚19种已知配体中哪一种作为主要的成骨分子，但越来越多的证据表明Wnt 1是骨形成背景下的关键参与者之一。事实上，尽管在早发性骨质疏松症患者中发现了杂合的WNT1突变，但纯合的WNT1突变会导致严重的儿童骨质疏松症，被归类为成骨不全XV型。IOBM产生的数据支持Wnt 1在骨代谢中的相关性，因为在成骨细胞中具有诱导性Wnt 1表达的小鼠模型显示由于成骨细胞活性增加而导致骨量显著增加。类似地，我们在具有成骨细胞特异性Wnt 1缺失的小鼠模型中观察到骨量减少和骨折风险增加，而Wnt 1依赖性早发性骨质疏松症的小鼠模型显示小梁和皮质骨量显著减少。其先前在患有XV型成骨不全症的患者中被鉴定。初步结果表明，这些动物不仅表现出减少的骨小梁和皮质骨质量，而且骨折的风险显着增加。在本提案中，将讨论（i）表型是否主要由骨基质质量受损或骨重塑改变引起，（ii）哪些下游途径介导Wnt 1的骨合成代谢作用，以及（iii）特立帕肽或抗吸收治疗是否会对表型产生积极影响。预期的结果不仅可以优化WNT1突变患者的治疗，还可以为开发治疗不同骨骼疾病的新治疗方案奠定基础。