Resistome in biogas plants (RiB) - Can biogas plants help to reduce the global spread of resistance?

沼气厂中的耐药性 (RiB) - 沼气厂能否帮助减少耐药性的全球传播？

• 批准号: 408898598
• 负责人: Dr. Christian Brandt
• 金额: --
• 依托单位: Department of Molecular Biology
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/408898598?language=en
• 关键词: Resistome; biogas; plants; RiB; help

The spread of antimicrobial resistance has been identified as one of the greatest threats to public health. Various factors, such as antibiotic consumption and antibiotic control in human and industrial use, as well as wastewater treatment and waste management play a cruicial role in the dissemination of antibiotic resistance. Mobile genetic elements, such as plasmids, gene cassettes and / or integrons on which most of the resistance genes are located, are readily transmissible resistance gene reservoirs, which also allow gene exchange between non-pathogenic and pathogenic bacterial species and vice versa. This has been increasingly observed in sewage treatment plants. The aim of this work is to investigate whether microbial diversity and density in biogas plants promote the transmission and distribution dynamics of resistance genes or whether the e. g. occasionally extreme fermentation conditions such as increased ammonium content, thermal pre-treatment, and elevated process temperatures prevents transfer or uptake of mobile DNA elements. For this purpose, the resistome of influence and effluence of various biogas processes will be analyzed using High-throughput and Long-Read Sequencing Technology.

抗菌素耐药性的传播已被确定为对公共卫生的最大威胁之一。各种因素，如人类和工业使用中的抗生素消费和抗生素控制，以及废水处理和废物管理，在抗生素耐药性的传播中起着至关重要的作用。大多数抗性基因所在的质粒、基因盒和/或整合子等可移动的遗传元件是易于传播的抗性基因储存库，也允许在非致病性和致病性细菌物种之间进行基因交换，反之亦然。这种情况在污水处理厂日益明显。这项工作的目的是研究沼气植物中的微生物多样性和密度是否促进了抗性基因的传播和分布动态，或者是否偶尔的极端发酵条件，如增加的铵含量，热预处理和升高的工艺温度阻止了移动DNA元素的转移或吸收。为此，将使用高通量和长读测序技术分析各种沼气过程的影响和流出的抗性组。

项目成果

Assessing genetic diversity and similarity of 435 KPC-carrying plasmids

• DOI: 10.1038/s41598-019-47758-5
• 发表时间: 2019-08-02
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Brandt, Christian;Viehweger, Adrian;Makarewicz, Oliwia
• 通讯作者: Makarewicz, Oliwia

Addressing dereplication crisis: Taxonomy-free reduction of massive genome collections using embeddings of protein content

解决重复删除危机：使用蛋白质内容嵌入对大量基因组集合进行无分类减少

• DOI: 10.1101/855262
• 期刊: bioRxiv
• 作者: A Viehweger;M Hölzer;​ C Brandt
• 通讯作者: ​ C Brandt