Study of RNA polymerase dynamics during elongation with cryo-electron tomography

在这个应用程序中，我们想研究的RNA聚合酶的动力学在延长与冷冻电子断层扫描。我们的目标是可视化延伸过程中RNA聚合酶和相关大分子的组织、相互作用和构象。我们希望在酵母和支原体细胞中进行这种分析，因为真核和细菌系统提供互补信息。冷冻电子断层成像应该在细胞背景下自然地可视化转录。我们预计实现一个决议，足以机械地解释聚合酶的构象状态，以及在延伸过程中的酶与其他因素的相互作用。

With this application we would like to study the RNA polymerase dynamics during elongation with cryo-electron tomography. We aim to visualize the organization, interactions and conformation of the RNA polymerase and associated macromolecules during elongation. We would like to perform this analysis in yeast and Mycoplasma cells, since the eukaryotic and bacterial systems provide complementary information. The cryo-electron tomograms should visualize the transcription natively in the cellular context. We anticipate achieving a resolution sufficient to mechanistically explain the polymerase conformational states, as well as the interactions of the enzyme with other factors during elongation.