Study of RNA polymerase dynamics during elongation with cryo-electron tomography

利用冷冻电子断层扫描技术研究 RNA 聚合酶在延伸过程中的动力学

• 批准号: 413805250
• 负责人: Professor Dr. Achilleas Frangakis
• 金额: --
• 依托单位: Institut für Biophysik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/413805250?language=en
• 关键词: Study; RNA; polymerase; dynamics; during

With this application we would like to study the RNA polymerase dynamics during elongation with cryo-electron tomography. We aim to visualize the organization, interactions and conformation of the RNA polymerase and associated macromolecules during elongation. We would like to perform this analysis in yeast and Mycoplasma cells, since the eukaryotic and bacterial systems provide complementary information. The cryo-electron tomograms should visualize the transcription natively in the cellular context. We anticipate achieving a resolution sufficient to mechanistically explain the polymerase conformational states, as well as the interactions of the enzyme with other factors during elongation.

通过这一应用，我们希望用冷冻电子断层扫描来研究RNA聚合酶在伸长过程中的动力学。我们的目标是可视化RNA聚合酶和相关大分子在伸长过程中的组织、相互作用和构象。我们希望在酵母和支原体细胞中进行这种分析，因为真核细胞和细菌系统提供了互补的信息。冷冻电子断层图像应该在细胞环境中自然地显示转录。我们期望达到一个足以从机械上解释聚合酶构象状态的分辨率，以及在伸长过程中酶与其他因素的相互作用。