ラット慢性低灌流状態モデルの脳循環代謝と白質の病態についての検討

慢性低灌注大鼠模型脑循环代谢及白质病理学检查

基本信息

  • 批准号:
    08770480
  • 负责人:
  • 金额:
    $ 0.7万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Encouragement of Young Scientists (A)
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 无数据
  • 项目状态:
    已结题

项目摘要

(1)オートラジオグラムによる局所脳血流、代謝の検討虚血作成後2日,1週,4週および8週後に局所脳血流量と局所脳ブドウ糖利用率を測定した。局所脳血流量では、2日後で皮質、白質、基底核領域において対照の33-58%まで低下し、2日から1週後まで持続した。1-4週後では各領域において回復傾向が見られたが、脳梁、内包で50%程度で低下したままであった。8週後では、脳梁、内包では70-80%程度の回復であったが、他の領域ではほぼ90%以上回復した。局所脳ブドウ糖利用率では、2日後で皮質、白質、基底核領域において対照の56-90%まで低下し、1週後には39-69%まで低下した。その後徐々に回復傾向を示し、4週後にはほぼ対照の90-100%まで回復し、8週後でも同様であった。(2)脳循環代謝と病理所見との比較検討虚血作成後2日、1週、4週および8週後に、4%paraformaldehydeで灌流固定を行ない、白質粗鬆化の評価は、Kluver-Barrera染色で行なった。虚血作成1週後より脳梁、内包等の白質で髄鞘の乱れが観察された。4週後では同部位に空胞形成が観察された。皮質では組織的形態は保たれていた。脳梁、内包等の白質の脳血流は4週後で50%程度で、梗塞に至らない程度の血流低下が1週から4週以上続くことが病理変化を起こしていると考えられた。(3)免疫病理学的検討虚血作成後2日、1週、2週、4週および8週後に、4%paraformaldehydeで灌流固定を行ない、白質におけるDNA断片化の有無についてTUNEL法で検討したところ、虚血1週後よりTUNEL陽性細胞が見られた。白質病変進展にグリア細胞のDNA断片化の関与が示唆された。
(1) the blood flow, blood flow and glucose utilization ratio of the bureau were measured 2 days after the completion of the blood flow test. After 2 days, the blood flow rate of the bureau was 33-58% lower than that of the skin, white and basal nuclear fields, and remained low after 2 days. After 1-4 years, all fields have returned to the level of 50% lower than before. At the end of 8 years, the degree of return is 70-80%, and that in other fields is more than 90%. After 2 days, the utilization rate of sugar was low. After 2 days, 56-90% of the sugar utilization rate was low, and 39-69% after 1 year. After 4 hours, please give a picture of 90% to 100% of the time. After 8 years, you will feel the same way. (2) after 2 days, 1 week and 4 weeks after the formation of deficiency blood in environmental pathology, 4%paraformaldehyde perfusion fixation, leukoplasization and Kluver-Barrera staining were performed. After the deficiency blood was made into 1 year, the white scabbard, such as the beam, the inner bag, and so on, were disorderly observed. (4) the empty cells in the same part of the body formed after 4 weeks. The shape of the skin tissue is closely related to that of the tissue. The blood flow of the girder, inner bag, etc., 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 50%, 4%, 4%, 4%, 4%, 4%, 4%, (3) in immunopathology, 2 days, 1 week, 2 days, 4 days after the operation of deficiency blood, 4%paraformaldehyde perfusion and fixation, DNA fragmentation, TUNEL method, and after 1 week, TUNEL, sexual cell infection. The progression of leukoplasias is characterized by DNA fragmentation and instigation.

项目成果

期刊论文数量(0)
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