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造血器腫瘍の増殖と分化におけるInterleukin-10の役割

IL-10在造血肿瘤增殖和分化中的作用

基本信息

批准号：
08770559
负责人：
大杉夕子
金额：
$ 0.58万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-08770559/
关键词：
Interleukin-10 白血病造血器腫瘍小児骨髄ストローマ細胞

项目摘要

小児における造血器腫瘍細胞とそれらの主たる増殖の場である骨髄のstroma細胞の両面からIL-10の造血器腫瘍の増殖,分化に対する役割について調べた.【方法と結果】白血病細胞株17種,白血病及び悪性リンパ種の臨床検体70例の腫瘍細胞を用いてRTPCR法によりIL-10,IL-10receptor(R)の発現を調べたところ,それぞれ1種,9例,2種,14例で発現が見られた.また造血器腫瘍細胞の増殖部位である骨髄stroma細胞ではIL-10のみ発現がみられた.IL-10発現例についてはELISA法にても細胞からのIL-10の分泌を確認し,これはTPA刺激により増強された.次に,IL-10,IL-10Rが発現している一部の細胞株を用いて,IL-10,抗IL-10中和抗体と共培養したときの腫瘍細胞の増強,分化,及びstroma細胞への接着(PKH26蛍光色素による生体染色)について検討したがIL-10は増殖,分化に直接的な影響は及ぼさず,stroma細胞への接着に関してはIL-10にて抑制,抗IL-10中和抗体添加にて増強を認めた.【結論】IL-10は腫瘍細胞の増殖,分化には関与を認めず,stroma細胞との接着に影響を及ぼすと考えられた.

There are 17 kinds of leukemic cell lines, 70 cases of leukemic and infectious leukemia cell lines, 70 cases of leukemic cell lines, 70 cases of leukemic and infectious leukemia cell lines. [methods] 17 kinds of leukemic cell lines, 70 cases of leukemic cells and 70 cases of leukemic cell lines were isolated from peripheral blood mononuclear cells. [methods] 17 kinds of leukemic cell lines, 70 cases of leukemic and atypical leukemia cell lines were detected by RTPCR method. [methods] 17 kinds of leukemic cell lines, 70 cases of leukemic and infectious leukemia cell lines were detected by RTPCR method. [methods] 17 kinds of leukemic cell lines, 70 cases of leukemic and infectious leukemia cell lines were selected. [methods] there were 17 kinds of leukemic cell lines, 70 cases of leukemic and infectious leukemia cell lines, 70 cases of leukemic cells and 70 cases of leukemic cell lines. [methods] A total of 70 cases of In IL-10receptor (R), there were 1, 9, 2, and 14 cases. The hematopoietic apparatus, colony site, bone stroma cell, IL-10 cell, IL-10, IL-10 and IL-10, respectively. In the second stage, IL-10,IL-10R showed that one cell line was cultured with anti-IL-10 neutralizing antibody (IL-10). The cells were strong, differentiated, and stroma cells were then stained with PKH26 light pigments. IL-10 colonization and differentiation were performed directly. Stroma cell cycle followed by IL-10 inhibition, anti-IL-10 neutralization antibody was added to strengthen recognition. [results] IL-10 cells colonized, differentiated cells and identified cells, stroma cells followed by shadow and mouse tests.