Presynaptic ATP dynamics

突触前 ATP 动力学

• 批准号: 416250083
• 负责人: Professor Dr. Stefan Hallermann
• 金额: --
• 依托单位: Carl-Ludwig-Institut für Physiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/416250083?language=en
• 关键词: Presynaptic; ATP; dynamics

Sustaining synaptic transmission over long periods of time is crucial for the function of the nervous system. The proteins mediating presynaptic neurotransmitter release are being intensely investi-gated. However, the energy homeostasis of the presynapse is less well understood. To address this fundamental question, we will combine high-resolution time-lapse imaging of ATP levels and other metabolites with electrophysiological techniques at cerebellar mossy fiber boutons in acute brain slices. The project has the potential to revealed the mechanisms controlling energy homeo-stasis of the presynapse and potential energetic constraints of transmitter release.

长时间维持突触传递对神经系统的功能至关重要。突触前神经递质释放的调节蛋白正受到广泛的研究。然而，突触前的能量稳态还不太清楚。为了解决这个基本问题，我们将结合联合收割机高分辨率的ATP水平和其他代谢物的延时成像与电生理技术在小脑苔藓纤维结急性脑切片。该项目有可能揭示控制突触前能量稳态的机制和递质释放的潜在能量限制。