Molecular mechanisms of high-frequency transmission at a central synapse

中央突触高频传输的分子机制

• 批准号: 204189369
• 负责人: Professor Dr. Stefan Hallermann
• 金额: --
• 依托单位: Carl-Ludwig-Institut für Physiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/204189369?language=en
• 关键词: Molecular; mechanisms; frequency; transmission; central

Rapid and precise information processing in our nervous system is based on the synaptic transmission between nerve cells. The influx of calcium ions triggers the fusion of transmitter filled vesicles at specialized presynaptic terminals. Although many of the underlying proteins are known, their regulation via calcium and their temporal and spatial interaction is up to now insufficiently understood. This is in particular the case because a direct access to the presynaptic terminal has been established at only some synapse types. In this project, cerebellar mossy fiber boutons (cMFBs) will therefore be investigated in acute brain slices of mice. These synapses are ideally suited to investigate high-frequency transmission, because they can transmit frequencies up to almost the Kilo-Hertz-range. To reveal the molecular mechanisms of high-frequency synaptic transmission, innovative high-resolution electrophysiological as well as optical methods will be combined at cMFBs. The results will be important for our understanding of rapid information processing in our central nervous system.

在我们的神经系统中，快速而精确的信息处理是基于神经细胞之间的突触传递。钙离子的流入触发了突触前末端的递质填充囊泡的融合。虽然许多潜在的蛋白质是已知的，但它们通过钙的调节和它们的时间和空间相互作用到目前为止还没有充分了解。这是特别的情况，因为直接进入突触前终端已经建立在一些突触类型。因此，本项目将在小鼠急性脑切片中研究小脑苔藓纤维钮扣（cMFBs）。这些突触非常适合研究高频传输，因为它们可以传输几乎高达千赫兹的频率。为了揭示高频突触传递的分子机制，创新的高分辨率电生理和光学方法将在cmfb结合起来。这一结果将对我们理解中枢神经系统的快速信息处理具有重要意义。

项目成果

State and location dependence of action potential metabolic cost in cortical pyramidal neurons

• DOI: 10.1038/nn.3132
• 发表时间: 2012-07-01
• 期刊: NATURE NEUROSCIENCE
• 影响因子: 25
• 作者: Hallermann, Stefan;de Kock, Christiaan P. J.;Kole, Maarten H. P.
• 通讯作者: Kole, Maarten H. P.

Apparent calcium dependence of vesicle recruitment

囊泡募集的明显钙依赖性

• DOI: 10.1113/jp275911
• 发表时间: 2018
• 期刊: The Journal of Physiology
• 作者: Ritzau-Jost A;Jablonski L;Viotti J;Lipstein N;Eilers J;Hallermann S
• 通讯作者: Hallermann S

Ultrafast Action Potentials Mediate Kilohertz Signaling at a Central Synapse

• DOI: 10.1016/j.neuron.2014.08.036
• 发表时间: 2014-10-01
• 期刊: NEURON
• 影响因子: 16.2
• 作者: Ritzau-Jost, Andreas;Delvendahl, Igor;Hallermann, Stefan
• 通讯作者: Hallermann, Stefan

NMDA Receptors Amplify Mossy Fiber Synaptic Inputs at Frequencies up to at Least 750 Hz in Cerebellar Granule Cells

• DOI: 10.1002/syn.21898
• 发表时间: 2016-07-01
• 期刊: SYNAPSE
• 影响因子: 2.3
• 作者: Baade, Carolin;Byczkowicz, Niklas;Hallermann, Stefan
• 通讯作者: Hallermann, Stefan