Function of axonal hyperpolarization activated currents

轴突超极化激活电流的功能

• 批准号: 317816582
• 负责人: Professor Dr. Stefan Hallermann
• 金额: --
• 依托单位: Carl-Ludwig-Institut für Physiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/317816582?language=en
• 关键词: Function; axonal; hyperpolarization; activated; currents

Cerebellar mossy fibers offer the chance to analyze myelinated central axons with direct patch clamp recordings at en-passant presynaptic boutons. Cerebellar mossy fibers boutons (cMFBs) generate ultrafast action potentials (half duration 100 µs), which can occur at frequencies higher than 1 kHz. Furthermore, cMFBs exhibit a prominent hyperpolarization activated current (I_h). Despite intense research the function of I_h in the axon remains largely unclear. Therefore, cMFBs offer the unique chance to directly investigate the function of I_h in axons. In the proposed project the hypotheses will be tested that I_h facilitates the generation of high-frequency action potentials, accelerates the speed of action potential propagation, and via modulation by Epinephrine regulates the function of axons. To investigate these hypotheses advanced electrophysiological techniques will mainly be used. The expected results are of general interest and could contribute to a better understanding of diseases, in which the propagation of action potentials in axons is impaired.

小脑苔藓纤维提供了机会，分析有髓鞘的中央轴突直接膜片钳记录在en-passant突触前扣。小脑苔藓纤维结（cMFB）产生超快动作电位（半持续时间100 µs），其可以在高于1 kHz的频率下发生。此外，cMFB还具有明显的超极化激活电流（I_h）。尽管有大量的研究，I_h在轴突中的功能仍不清楚。因此，cMFB为直接研究I_h在轴突中的功能提供了独特的机会。本研究拟验证I_h促进高频动作电位的产生，加快动作电位的传播速度，并通过肾上腺素调节轴突功能的假说。为了研究这些假设，将主要使用先进的电生理技术。预期的结果是普遍的兴趣，并可能有助于更好地了解疾病，其中轴突中的动作电位的传播受损。

项目成果

Gradients in the mammalian cerebellar cortex enable Fourier-like transformation and improve storing capacity

哺乳动物小脑皮层中的梯度能够实现类傅里叶变换并提高存储能力

• DOI: 10.7554/elife.51771
• 发表时间: 2020
• 期刊: eLife
• 影响因子: 7.7
• 作者: Straub I;Witter L;Eshra A;Hoidis M;Byczkowicz N;Maas S;Delvendahl I;Dorgans K;Savier E;Bechmann I;Krueger M;Isope P;Hallermann S
• 通讯作者: Hallermann S

HCN Channel-mediated neuromodulation can control action potential velocity and fidelity in central axons

• DOI: 10.7554/elife.42766
• 发表时间: 2019-09-09
• 期刊: ELIFE
• 影响因子: 7.7
• 作者: Byczkowicz, Niklas;Eshra, Abdelmoneim;Hallermann, Stefan
• 通讯作者: Hallermann, Stefan