胆道系悪性腫瘍の遺伝子及び癌抑制遺伝子の解析

胆道恶性肿瘤基因及抑癌基因分析

• 批准号: 08770987
• 负责人: 須藤 隆之
• 金额: $ 0.51万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-08770987/
• 关键词: p53; APC; ras; DCC; 胆管癌; 乳頭部癌; SSCP; LOH

胆道系悪性腫瘍の遺伝子及び癌抑制遺伝子の解析対象:胆管癌43例、乳頭部癌16例を用いてp53,APC,ras,DCCの解析を行った。方法:p53,APC,rasのpoint mutationは、PCR-SSCP法にて解析した。p53,APC,DCCの染色体の欠損は、PCR-LOH法にて解析した。結果1)p53mutation:胆管癌43例中14例(32.6%)、乳頭部癌16例中2例(12.5%)LOH:胆管癌17例中6例(35.3%)、乳頭部癌3例中2例(66.6%)2)APCmutation:胆管癌43例中1例(2.3%)、乳頭部癌16例中3例(18.8%)LOH:胆管癌12例中2例(16.7%)、乳頭部癌3例中2例(66.6%)3)rasmutation:胆管癌43例中8例(18.6%)、乳頭部癌16例中3例(18.8%)4)DCCLOH:胆管癌21例中4例(19.0%)乳頭部癌9例中1例(11.1%)以上の解析を行い、現在、mutatio症例のシークエンス中である。

43 cases of cholangiocarcinoma and 16 cases of breast cancer were analyzed with the p53 ~ APC ~ ras ~ DCC. Methods: p53 APC point mutation ras and PCR-SSCP method were used to analyze the samples. The chromosome of p53 APC DCC chromosome was deficient, and the PCR-LOH method was used to analyze the chromosome. Results 1) p53mutation: 14 of 43 cases of cholangiocarcinoma (32.6%), 2 of 16 cases of breast cancer (12.5%) LOH: 6 of 17 cases of cholangiocarcinoma (35.3%), 2 of 3 cases of breast cancer (66.6%) APCmutation: 1 of 43 cases of cholangiocarcinoma (2.3%), 3 of 16 cases of breast cancer (18.8%) LOH: 2 of 12 cases of cholangiocarcinoma (16.7%) Of the 3 cases of breast cancer, 2 cases (66.6%) 3) rasmutation: 8 cases (18.6%) of 43 cases of cholangiocarcinoma and 3 cases (18.8%) of 16 cases of breast cancer 4) DCCLOH: 4 cases (19.0%) of 21 cases of cholangiocarcinoma and 1 case (11.1%) of 9 cases of breast cancer.