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重金属の鼻粘膜細胞のシグナル伝達に与える影響

重金属对鼻粘膜细胞信号传导的影响

基本信息

批准号：
08771399
负责人：
加藤昌志
金额：
$ 0.7万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至无数据
项目状态：
已结题

项目摘要

1.スギ花粉症患者について花粉の飛散する約1ヵ月前(1月)、飛散前期(2月)、飛散中期(3月)、飛散後期(4月)および飛散終了後約1ヵ月(5月)の5回、血清および鼻腔洗浄液を採取し、これらの含まれている可溶性接着分子(可溶性ICAM-1)、可溶性VCAM-1、可溶性E-selectin、可溶性L-selectin)、およびIL-4を市販のELISAキットを用いて測定した。血清中の可溶性ICAM-1および可溶性VCM-1は飛散前期に有意に上昇し、可溶性L-selectinは逆に同時期に有意に低下した[Kato M,et al.Arch Otolaryngol (in press)]。一方、可溶性E-selectinは全期間を通してほとんど変化しなかった。鼻腔洗浄液中の可溶性ICAM-1は、飛散中期に最高値に達した[Kato M,et al.Allergy 51:128-132,1996]。鼻腔洗浄中の可溶性VCAM-1、可溶性E-selectin、可溶性L-selectinは測定下限値以下であった。さらに、血清および鼻腔洗浄液中のIL-4は飛散中期から飛散終了後約1ヵ月にかけて上昇し、飛散中期および飛散後期において血清中の可溶性ICAM-1と負の相関関係を示した[Kato M,et al.Ann Oto Rhino Laryn(in press)]。以上より、可溶性接着分子およびIL-4は自然飛散状態でのスギ花粉症の病態に深く関与している可能性が示唆された。2.大気汚染物質の一つである重金属(塩化第二水銀)が細胞の活性化にどのような影響を与えるかについて、特に細胞内シグナル伝達の初期段階である蛋白のチリシンリン酸化にターゲットをしぼって解析した。塩化第二水銀はin vivo[Pu M,Akand AA,Kato M,et al.J Cell Biochem 63:104-114,1996]およびin vitro[Pu M,Akhand A Anwarul,Kato M,et al.Oncogene (in press)]でSrc高発現マウス線維芽細胞のc-Srcの自己リン酸化を著しく促進した。これは塩化第二水銀が細胞を著しく活性化する可能性を示唆している。

1. The patients with pollinosis were divided into five groups about 1 month ago (January), early stage (February), middle stage (March) and late stage (April). After about 1 month (May), the serum samples were collected from the nasal lavage solution, which contained soluble ICAM-1, soluble VCAM-1, soluble E-selectin, and so on. Soluble L-selectin), IL-4 the market, ELISA the temperature, and determine the temperature by the temperature measurement. The serum levels of soluble ICAM-1 and soluble VCM-1 were intentionally elevated in the early stage, and the levels of soluble L-selectin were intentionally decreased in the same period [Kato MJ et al.Arch Otolaryngol (in press)]. One party, soluble E-selectin, the whole period of the whole period. The soluble ICAM-1 in nasal lavage fluid and the highest concentration in the middle stage of nasal lavage [Kato Mpene et al.Allergy 51Rut128-132 1996]. The lower limit of the determination of soluble VCAM-1, soluble E-selectin and soluble L-selectin in nasal washing was below the limit. The IL-4 in the nasal lavage fluid was dispersed in the middle and later stages of the powder, and the soluble ICAM-1 in the serum was detected in the middle and later stages of the powder. [Kato MJO et al.Ann Oto Rhino Laryn (in press)]. The above soluble molecules are followed by molecules such as "IL-4", "natural", "scattered", "hay fever", "deep" and "possibility". two。 In the first place of the dye, the heavy metal (the second water) was activated, the cell was activated, the heavy metal was activated, the cell was activated, and the cell was activated. The second water plant in vivo [Pu MMagneAkand AA,Kato MMaget al.J Cell Biochem 6314] in vitro [Pu Menera Akhand An Anwarul,Kato Mrecog et al.Oncogene (in press)] Src shows that it has been acidified by its own authors, c-Src. The second water cell is instigated by the possibility of activation.