Biophysical and molecular mechanisms of mean and variance adaptation in Drosophila auditory receptor neurons (A10*)

果蝇“耳朵”接受器中的机械感觉神经元执行两种机械独立的适应类型：对接受器偏转的平均值和方差的适应。我们将使用遗传工具(RNAi，MIMIC)来操纵潜在涉及的分子(如Trp和离子通道，马达蛋白)的功能，并使用双光子钙和电压成像或电生理学读出不同细胞间隔中由此产生的适应变化。苍蝇耳朵中从刺激到脉冲输出的转换的计算模型将作为分析工具和将实验结果整合到适应的功能模型中的脚手架。

Mechanosensory neurons in the Drosophila “ear” receiver perform two mechanistically independent types of adaptation: To the mean and the variance of receiver deflections. We will use genetic tools (RNAi, MiMIC) to manipulate the function of potentially involved molecules (e.g. TRP and ion channels, motor proteins) and readout resulting changes of adaptation in different cellular compartments using two-photon calcium- and voltage-imaging or electrophysiology. A computational model of the transformation from stimulus to spiking output in the fly ear will serve as an analysis tool and as a scaffold to integrate experimental results into a functional model of adaptation.