胎児期の低酸素性虚血性脳障害の予防に関する実験的研究

预防胎儿期缺氧缺血性脑损伤的实验研究

• 批准号: 09770863
• 负责人: 岩崎 信明
• 金额: $ 1.28万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09770863/
• 关键词: 動物実験モデル; 低酸素性虚血性脳症; 胎児期; proteolopid protein

胎児期の脳障害の成因を明らかすることを目的とし動物実験モデルを作成し、脳障害の指標として髄鞘化障害に注目し、myelin basic protein(MBP),proteolipid protein(PLP)に対する免疫染色法を開発し、髄鞘化障害に対して定量的に検討した。【方法】出生後0,7,14,21,28,35,42日のWister系正常仔ラットを体重測定後に断頭し脳を取り出した(対照群)。胎児期低酸素虚血負荷ラットは妊娠15日に片側の子宮動静脈の結紮離断し、妊娠16-18日の3日間、母胎への低酸素負荷(7%)を3時間施行した(負荷群)。子宮動脈結紮4時間前にデキサメサゾン0.1mg/kgを母ラット腹腔内に注射した(治療群)。妊娠22日に帝王切開で仔を娩出し、結紮部位からの3頭について、出生後21日目に開頭して脳を取り出し、ホルマリン固定後パラフィンで包埋した。抗MBP抗体または抗PLP抗体、ビオチン標識IgG抗体を反応させ、ABC法により免疫染色して、画像解析装置で部位毎の染色濃度を測定した。【結果及び考察】MBP,PLPは日齢7頃から染色され週齢とともに染色濃度は増加した^<1)>。負荷群と治療群はともに体重は対照群と比較して80-90%減少したが、脳重に有意差はみられなかった。HE染色、KB染色および抗MBP抗体・抗PLP抗体免疫染色で有意な変化は認められなかった。負荷群では抗GFAP染色で皮質、脳梁膨大部でGFAP陽性細胞の明らかな増加がみられた。治療群では明らかな増加はみられなかった。白質、基底核で有意な増加はなかった。【結論】抗MBP抗体、抗PLP抗体免疫染色は髄鞘化障害の判定に有効であった。皮質と脳梁膨大部が子宮動脈結紮+母胎低酸素負荷による負荷では脆弱である可能性が示唆された。デキザメサゾンの負荷前投与が脳障害の発症に予防効果がある可能性が考えられた。1)Hamano K,Takeya T,Iwasaki N.et al.A quantitative studyof the progress of myelination in the rat central nervous system, using the immunohistochemical method for proteolipid protein Developmental Brain Research.108:287-93,1998

The cause of fetal malfunction is clear that the animal is damaged in order to determine the quality of the animal, and the malfunction refers to the quantification of the sheath damage by immunostaining, myelin basic protein (MBP), proteolipid protein (PLP) immunostaining. [methods] after birth, 07pcg 14pr 21je 28je 35,42 days after birth, Wister normal offspring were tested for weight, and then their weight was measured and then they were taken out of the baby group. During the period of fetal hypoacidosis, fetal hypoacid deficiency, fetal Before the end of the child action 4, the mother of the 0.1mg/kg mother was injected intraperitoneally with the drug (treatment group). On the 22nd of pregnancy, the emperor cut open the baby to be delivered, and at the end of the pregnancy, the baby was removed from the baby at the end of the pregnancy. On the 21st day after birth, the baby was removed, and the baby was fixed and then buried. Anti-MBP antibody, anti-PLP antibody, anti-IgG antibody, anti-DNA antibody, anti-DNA antibody, [results and investigation] MBP,PLP was stained on the 7th day, and the staining intensity was increased by the addition of gt;. The weight of the lotus group is 80-90% less than that of the control group, and the weight is intentionally lower. HE staining, KB staining, anti-MBP antibody and anti-PLP antibody immunostaining. The lotus group, anti-GFAP staining, anti-GFAP, anti-DNA staining, anti-staining, anti-staining and anti-staining. To treat a group of people in a clear way and add a lot of money to it. Leukoplakia, basal ganglia, purposeful parasitosis and parasitosis. [results] the immune staining of anti-MBP antibody and anti-PLP antibody was used to determine the damage of sheath. The results of the exercise in the inflated part of the girder + maternal-fetal low-acid diet, maternal In order to prevent the disease, the possibility of the disease will be tested. 1) Hamano KMagna Takeya Trecinct Iwasaki N.et al.A quantitative studyof the progress of myelination in the rat central nervous system, using the immunohistochemical method for proteolipid protein Developmental Brain Research.108:287-93Cool 1998