Functional characterization of novel mechanisms regulating SERCA activity and Ca2+ homeostasis in D. melanogaster (P21*)

黑腹果蝇 SERCA 活性和 Ca2 稳态调节新机制的功能表征 (P21*)

• 批准号: 418262793
• 金额: --
• 依托单位: Arbeitsgruppe Zoologie-Entwicklungsbiologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/418262793?language=en
• 关键词: Functional; characterization; novel; mechanisms; regulating

The project P21 focuses on the mechanistic understanding of a novel process that regulates activity of the calcium ATPase (SERCA) within Ca2+ uptake membrane microdomains of the sarcoplasmic reticulum of flies. Impaired activity of the metalloprotease Nep4 significantly affected cardiac Ca2+ homeostasis and induced a strong arrhythmia phenotype. To understand the molecular mechanisms underlying the Nep4 mediated regulation of SERCA, they will (i) identify phenotype-relevant Nep4 peptide substrates, (ii) analyze the suborganellar localization, and (iii) determine the funciton and dynamics between Nep4 and SERCA in detail.

P21 项目侧重于对调节果蝇肌浆网 Ca2+ 吸收膜微域内钙 ATP 酶 (SERCA) 活性的新过程的机制理解。金属蛋白酶 Nep4 的活性受损会显着影响心脏 Ca2+ 稳态并诱导强烈的心律失常表型。为了了解 Nep4 介导的 SERCA 调节的分子机制，他们将 (i) 鉴定与表型相关的 Nep4 肽底物，(ii) 分析亚细胞器定位，以及 (iii) 详细确定 Nep4 和 SERCA 之间的功能和动力学。