The polyamine system in gender-related renoprotection (B05)

我们将研究多胺系统的变化如何影响阿基的性别依赖性易感性。为了认识到性别相关的多胺水平对阿基结果的影响，我们将使具有和不具有性腺切除术的雄性小鼠以及具有和不具有睾酮治疗的雌性小鼠暴露于肾IRI。将阻断这些动物中的多胺产生和分解，以探索阿基结果中的性别依赖性差异是否可以通过多胺系统的药理学抑制来减弱。此外，小鼠胚胎肾外植体和斑马鱼幼虫将被用作筛选工具，以研究睾酮如何干扰靶向多胺稳态的肾脏保护的前瞻性药物。

We will investigate how changes in the polyamine system contribute to the gender-dependent susceptibility to AKI. To recognize the influence of sex-related polyamine levels on the outcome of AKI, we will expose male mice with and without gonadectomy and female mice with and without testosterone treatment to renal IRI. Polyamine production and breakdown in these animals will be blocked to explore whether gender-dependent differences in the outcome of AKI can be attenuated by pharmacologic inhibition of the polyamine system. Furthermore, murine embryonic kidney explants and zebrafish larvae will be used as screening tools to investigate how testosterone interferes with prospective drugs targeting the homeostasis of polyamines for renoprotection.