Mechanisms of calcium homeostasis and signal integration in primary cilia (A22*)

初级纤毛中钙稳态和信号整合的机制 (A22*)

• 批准号: 418686646
• 金额: --
• 依托单位: Zentrum für Human- und Molekularbiologie (ZHMB)
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/418686646?language=en
• 关键词: Mechanisms; calcium; homeostasis; signal; integration

Primary cilia create unique signalling compartments of the eukaryotic cell by orchestrating signalling molecules in a small, refined space. Defects in putative Ca2+ channels play a major role in cilia-dependent disorders, such as polycystic kidney disease. The central goal of this project is to determine basic mechanisms of Ca2+ homeostasis in cilia and how they impact the signalling environment of cilia in health and disease. To this end we are combining advanced quantitative proteomics techniques with microscopic approaches using a novel cilia Ca2+ sensor to tackle these questions from multiple angles.

初级纤毛通过在一个小而精细的空间中编排信号分子，在真核细胞中创建独特的信号间隔。推测的钙通道缺陷在纤毛依赖性疾病，如多囊肾病中起主要作用。本项目的中心目标是确定纤毛中钙离子稳态的基本机制，以及它们如何影响纤毛在健康和疾病中的信号环境。为此，我们正在将先进的定量蛋白质组学技术与使用新型纤毛钙传感器的显微方法相结合，从多个角度解决这些问题。