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寄生虫・エキノコックスの終宿主免疫応答の解析と抗原解析による免疫誘導への可能性

寄生虫棘球蚴的最终宿主免疫反应分析以及通过抗原分析诱导免疫的可能性

基本信息

批准号：
11760210
负责人：
野中成晃
金额：
$ 1.41万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

終宿主におけるエキノコックス再感染時の獲得抵抗性を観察するために、虫卵形成異常を示すエキノコックスの分離株Aと正常な虫卵形成を示す分離株Bを用いて犬への感染実験を行った。再感染群には分離株Aを経口投与し、5週間後に分離株Bを経口投与した。対照群には分離株Aの投与は行わず、分離株Bのみを経口投与した。エキノコックス成虫に対するモノクローナル抗体、EmA9およびEmiで糞便内抗原の排出動態を観察したところ、再感染群の分離株B投与後の抗原排出量は対照群よりも抑制され、再感染時に分離株Bの定着が制限されたことが疑われた。また、実験感染後の回収虫体は両群とも大きな差がなかったが、再感染群では子宮に虫卵を包蔵しない虫体が多く含まれ、分離株Aの寄生のために、分離株Bの定着が阻害されたことを示唆する結果となった。分離株A,Bの回収数については、回収虫体のDNA分析を適用して算出する予定である。さらに、野外で捕獲したキツネよりエキノコックス成虫を回収し、成虫抗原を作成し、また、密度分離法によって成虫より虫卵を分離して虫卵抗原を作成し、スナネズミ由来の原頭節抗原とあわせて、各種エキノコックス抗原に対する血清抗体価を測定したところ、感染後に血清抗体価が上昇したが、両群間に顕著な差は認められなかった。糞便内IgAについても同様の解析を行う予定である。また、腸管の炎症反応の有無を見るために、血清急性相蛋白(CRP)の経時変化を観察したところ、感染後1週目にCRPの上昇が見られ、炎症が起こっていることが示唆された。なお、現在、経口免疫の効果判定法として、代替終宿主であるハムスターに原頭節の感染実験を行い、原頭節抗原、成虫抗原および虫卵抗原を用いたパイエル氏板および腸管膜リンパ節のリンパ球増殖反応の実験系を確立中である。

When the host was infected again, it was resistant to infection. The egg formation test showed that the isolated strain A was infected with normal egg formation, and the isolated strain B was infected with the dog virus. The reinfection group isolates strain An and B after 5 years of infection. According to the group, isolate strain A was injected into the line, and strain B was injected orally with strain B. The antibody of adult worms and the intrafecal antigen of EmA9 Emi were excreted by dynamic assay, the antigen excretion of reinfection group B and the antigen excretion of reinfection group B were determined according to the group inhibition test, and the reinfection time isolation strain B was confirmed by restriction assay. After infection, there was a large difference in the number of parasites, the eggs of the reinfection group, the eggs of the reinfection group, the parasitic parasites of strain An and strain B. the results showed that the results showed that the parasites of strain An and strain B were infected. The isolated strain A, B, and DNA analysis were used to calculate the number of predetermined samples. The eggs were isolated from the eggs of the adults, the eggs were separated from the eggs of the adults, the eggs of the eggs were separated from the eggs of the adults, the eggs of the eggs were separated from the eggs of the adults, the eggs were separated from the eggs of the adult worms, and the origin of the antigen was detected. The original antigens were not detected, and the serum antibodies of all kinds of antigens were determined. After infection, the serum antibodies were detected by Elisa, and the difference between groups was detected. The IgA in the stool will be parsed in the same way. The serum acute phase protein (CRP), serum acute phase protein (CRP), serum acute phase protein (APS), serum acute phase protein (ACP), serum acute phase protein (CRP), serum acute phase protein (APS), serum acute phase protein (APA), serum acute phase protein (APS), serum acute phase protein (APA), serum acute phase protein (APA), serum acute phase protein (APS), serum acute phase protein (APA), serum acute phase protein (APS), serum acute phase protein (APA), serum acute phase protein (APA) and inflammatory response were observed. The method of determining the results of oral immunization, present and oral immunization, instead of the host virus, the original virus antigen, the adult worm antigen, the egg antigen, the tube membrane, the tube membrane, the ball, the ball, the egg antigen, the egg antigen, the host, the host, the original antigen, the adult worm and egg antigen.