Tc-99m MIBIを用いた放射線照射のヒトがん細胞の代謝におよぼす影響の検討

使用 Tc-99m MIBI 检查放射线照射对人类癌细胞代谢的影响

• 批准号: 11770520
• 负责人: 古田 雅也
• 金额: $ 0.58万
• 依托单位: Dokkyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11770520/
• 关键词: 放射線; Tc-99m MIBI; ヒトがん細胞; DiOC6(3); ミトコンドリア膜電位; カフェイン; X線照射; 放射線生物学; ミトコンドリア代謝; 放射線障害; 回復現象

1.放射線照射後のTc-99m MIBI集積変化 A549細胞では放射線照射後にTc-99m hexakis-2-methoxy-isbutyl-isonitrile[MIBI]集積が亢進した.その集積は照射直後-2時間と6-12時間後に2峰性の増加を示した.さらに放射線感受性の異なる2種のがん細胞株で検討したが,やはり放射線照射後にTc-99m MIBI集積が増加した.2.フローサイトメトリによるミトコンドリア膜電位の解析 フローサイトメトリによる3,3′-dihexyloxacarbocyanine[DiOC.(3)]を用いた解析から,A549細胞のミトコンドリア膜電位は照射直後および4-12時間後の2峰性の増大を示し,膜電位の変化がTc-99m MIBI集積の変化の主因であることが確認された.3.放射線照射後の細胞周期の変化とミトコンドリア膜電位の関連 フローサイトメトリによる解析からA549細胞は照射4-8時間後にG2/M期細胞が増加した(G2ブロック).上記のTc-99m MIBI集積の第2ピークはこの照射後のG2ブロックの時期とほぼ一致していたことから,細胞周期をカフェイン(照射後のG2ブロックを抑制することが知られている)を用いて変更し,ミトコンドリア膜電位への影響を検討した,その結果,G2ブロックが消失しても膜電位にはほとんど変化がみられず,膜電位は細胞周期に依存しないことが判明した.4.まとめ がん細胞は放射線照射後にTc-99m MIBI集積が増加し,集積の増加はミトコンドリア膜電位の増大による.膜電位の増大は細胞周期に依存しない.またミトコンドリア膜電位の増大は照射直後から生じることから,こめ膜電位の変化は照射後極めて早期に生じる細胞代謝上の反応と考えられた.研究成果の一部は平成12年11月に日本放射線腫瘍学会第13回学術大会にて発表した.

1. After radiation, the Tc-99m MIBI concentration increased significantly in A549 cells. After radiation, the Tc-99m hexakis-2-methoxy-isbutyl-isonitrile [MIBI] cluster increased. Two peaks were detected immediately after irradiation-2 hours after 6-12 hours and then 2 peaks. The cell lines of the two strains were sensitive to radiation, and the Tc-99m MIBI cluster was added after radiation. 2. The temperature of the membrane potential is very high, and the temperature of the film potential is very high. The temperature of the film potential is very high, and the temperature of the film potential is very high. The temperature of the film potential is very high, and the temperature of the film potential is very high. The temperature of the film potential is determined by the temperature analysis of dihexyloxacarboboxylic acid [DiOC. (3)]. The cell temperature of the A549 cell, the temperature of the film, the temperature of the film, the temperature, the temperature, the temperature, After radiation, the cell cycle was changed, the cell cycle was changed, the membrane potential was analyzed, and the cell cycle of A549 cells was analyzed. After 4-8 irradiation, G _ 2max M-phase cells were added to G _ 2 phase. After exposure to the second cycle of the previous Tc-99m MIBI cluster, the G2 cycle cycle after irradiation, the cell cycle cycle response, the cell cycle response, the cell cycle, the cell cycle, the temperature, the temperature, The G2 cell cycle disappears, the membrane potential changes, and the cell cycle of the membrane potential depends on each other. 4. After radiation exposure, the Tc-99m MIBI will be increased, and the film potential will be increased. The membrane potential is highly dependent on the cell cycle. When the membrane potential was irradiated directly, it was found that there was no significant difference between the two groups. After direct irradiation, there was a negative reaction in the early stage of the cell generation. The research results are published in Heicheng, November 12, the 13th round of the academic conference of the Japan Radiology Association.