Target cis elements of the RNA-binding protein Arid5a in inflammatory cytokine-based diseases (B16*)

炎症细胞因子疾病中 RNA 结合蛋白 Arid5a 的靶标顺式元件 (B16*)

• 批准号: 423465262
• 金额: --
• 依托单位: Institut für Molekulare Biowissenschaften
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/423465262?language=en
• 关键词: Target; cis; elements; RNA; binding

Tight control of gene expression is particularly important during development and immune response. The post-transcriptional gene regulation comprises the control over mRNA stability and their translation into gene products. mRNAs harbor cis-regulatory elements in their 3´- untranslated regions that are targeted by RNA-binding proteins (RBPs) and subsequently decide about the transcript fate. In this context, mRNA degradation, e.g., has already been studied for two decades. We will investigate the novel RBP Arid5a and its role in stabilizing, i.e. up-regulating levels of target mRNAs coding for proteins involved in cytokine production during immune responses. We will define target cis elements of Arid5a and obtain atom-resolved pictures of RNA-protein complexes that will reveal mechanisms of Arid5a-based gene regulation and its role in cytokine-based diseases.

基因表达的严格控制在发育和免疫反应过程中尤为重要。转录后基因调控包括控制 mRNA 稳定性及其翻译成基因产物。 mRNA 在其 3´-非翻译区中含有顺式调控元件，这些元件是 RNA 结合蛋白 (RBP) 的目标，随后决定转录物的命运。在这种背景下，mRNA 降解等问题已经被研究了二十年。我们将研究新型 RBP Arid5a 及其在稳定（即上调免疫反应期间参与细胞因子产生的蛋白质编码的靶标 mRNA 水平）中的作用。我们将定义 Arid5a 的靶顺式元件，并获得 RNA-蛋白质复合物的原子分辨图像，这将揭示基于 Arid5a 的基因调控机制及其在细胞因子疾病中的作用。