Enzyme cooperativity in the biosynthesis of antibiotics and their cellular target structures (B06)

酶的协同作用对于许多生物合成过程是重要的。本计画将针对抗生素的各种生物合成机制或其细胞目标结构，研究酶的协同作用。我们将研究协同性在细胞中是如何组织的，重点是三个主要系统中酶之间的相互作用：1）PKS/NRPS生物合成中的磷酸泛酰巯基乙胺转移酶和载体蛋白，2）萜烯生物合成中的细胞色素P450和氧化还原伴侣蛋白，3）细胞壁生物合成中的丙氨酸消旋酶和D-Ala-D-Ala连接酶。

The cooperativity of enzymes is important for many biosynthetic processes. In this project, enzyme cooperativity will be studied for various biosynthetic machineries of antibiotics or their cellular target structures. We will investigate how cooperativity is organized in the cell focusing on the interaction between enzymes in three main systems: 1) phosphopantetheinyl transferases and carrier proteins in PKS/NRPS biosynthesis, 2) cytochromes P450 and redox partner proteins in terpene biosynthesis, and 3) alanine racemase and D-Ala-D-Ala ligase in cell wall biosynthesis.