Structures of cellular machineries relevant for antibiotic synthesis and action (B08)

点击翻译按钮获取中文摘要

In the first part of this project, we will characterize the interactions between subunits of a multi-enzyme complex that synthesizes the glycopeptide antibiotic balhimycin in Amycolatopsis balhimycina. In the second part, we will identify substrate specificities and enzymatic mechanisms of enzyme complexes that catalyze lipid II amidation in Staphylococcus aureus and A. balhimycina. Both objectives will be pursued using protein crystallography and associated biophysical techniques as well as functional assays. The overall goal of the proposed work is to advance an understanding of enzymatic mechanisms relevant for the biosynthesis and function of antibiotics.