Structures of cellular machineries relevant for antibiotic synthesis and action (B08)

与抗生素合成和作用相关的细胞机器结构（B08）

• 批准号: 425877271
• 金额: --
• 依托单位: Interfakultäres Institut für Biochemie (IFIB)
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/425877271?language=en
• 关键词: Structures; cellular; machineries; relevant; antibiotic

In the first part of this project, we will characterize the interactions between subunits of a multi-enzyme complex that synthesizes the glycopeptide antibiotic balhimycin in Amycolatopsis balhimycina. In the second part, we will identify substrate specificities and enzymatic mechanisms of enzyme complexes that catalyze lipid II amidation in Staphylococcus aureus and A. balhimycina. Both objectives will be pursued using protein crystallography and associated biophysical techniques as well as functional assays. The overall goal of the proposed work is to advance an understanding of enzymatic mechanisms relevant for the biosynthesis and function of antibiotics.

在这个项目的第一部分，我们将表征多酶复合物的亚基之间的相互作用，该复合物在拟无枝酸菌balhimycina中合成糖肽抗生素balhimycin。在第二部分中，我们将确定底物特异性和酶复合物的酶催化脂质II酰胺化在金黄色葡萄球菌和A。Balhimycina。这两个目标将采用蛋白质晶体学和相关的生物物理技术以及功能测定。拟议工作的总体目标是促进对抗生素生物合成和功能相关的酶机制的理解。