Shrimp Rhodopsins as new far-red absorbing optogenetic tools

虾视紫红质作为新的远红光吸收光遗传学工具

• 批准号: 426019636
• 负责人: Professor Dr. Peter Hegemann
• 金额: --
• 依托单位: Institut für Physiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/426019636?language=en
• 关键词: Shrimp; Rhodopsins; new; far; red

Red light is most attractive for stimulation of optogenetic actuators and reporter systems, since it is less harmful than short-wavelength light, penetrates well through tissue and does spectrally not interfere with most other available optical tools. Among the animal rhodopsins with the highest sensitivity to red light in nature are the mantis shrimp’s red sensitive rhodopsins that can be activated with near-infrared light beyond 700 nm. We will characterize the 9 already sequenced rhodopsins of the Shrimp Neogonodactylus oerstedii and variants of related species. We will overcome the poor expression of invertebrate rhodopsin by codon optimization and tailoring of the C- and N-termini and characterize the biophysical properties of the recombinant proteins. We will determine and modulate the G-protein coupling and use these rhodopsins for the modification of signaling cascades in neurons for non-invasive deep tissue activation.

红光对于光遗传学致动器和报告系统的刺激是最有吸引力的，因为它比短波长光危害小，很好地穿透组织，并且在光谱上不干扰大多数其他可用的光学工具。在自然界中对红光具有最高敏感性的动物视紫红质中，螳螂虾的红色敏感视紫红质可以被超过700 nm的近红外光激活。我们将描述9个已经测序的视紫红质的虾Neogonodactylus oerstedii和相关物种的变种。我们将克服无脊椎动物视紫红质的密码子优化和剪裁的C-和N-末端和生物物理特性的重组蛋白的表达差。我们将确定和调节G蛋白偶联，并使用这些视紫红质修饰神经元中的信号级联，用于非侵入性深部组织激活。